QIAsprint solution for DNA isolation from blood

The QIAsprint solution for genomic DNA isolation from blood is a modular magnetic-bead based system for high troughput DNA isolation. The system combines the flexibility of modular design with the power of magnetic-bead–based purification, enabling fully automated processing on the QIAsprint Connect without manual intervention including an optional fully automated on board-lysis step.

The solution consists of dedicated modules that can be flexibly combined or exchanged depending on sample type and workflow needs. For genomic DNA isolation from blood we recommend the following modules:

• QIAsprint DNA Blood PrepSet (384): Designed for efficient pretreatment of tissue and cell samples, this module includes Buffer AL for cell lysis, and Proteinase K for thorough protein digestion.
• QIAsprint Essential Kit A (384): Provides the magnetic beads and wash buffers required for DNA purification. The bead-based technology ensures high yield, reproducibility, and recovery of high-quality DNA.

All modules are designed and optimized for fully automated processing of up to 384 samples on the QIAsprint Connect. The platform ensures minimal hands-on time and reproducible performance.

The QIAsprint DNA Blood workflow delivers DNA yields comparable to or higher than those obtained with other leading magnetic-bead–based purification systems, such as MagMAX kits. This performance is consistent even with challenging sample types such as fibrous tissues or fatty (see Comparable DNA recovery).

Digital PCR analysis demonstrates higher DNA concentrations in samples processed with the QIAsprint instrument (see Higher DNA concentration).

The QIAsprint DNA Blood workflow uses a modular approach for efficient and flexible DNA purification on the QIAsprint Connect. Sample pretreatment is performed with the QIAsprint DNA Blood PrepSet (384), where Buffer AL and Proteinase K digestion enables complete lysis of the blood sample. The QIAsprint Essential Kit A (384) then provides the magnetic beads and buffers required for the automated bind–wash–elute steps on the QIAsprint Connect. During processing, DNA binds to the magnetic beads, contaminants are efficiently washed away, and high-quality DNA is eluted for downstream analysis.

If the workflow needs to be adapted for other sample types or input materials, the flexibility of the QIAsprint modular system allows users to combine the QIAsprint DNA Blood PrepSet with other compatible Essential Kits (Link to Essentail kit page). For detailed guidance, refer to the Essential Kit page and the QIAsprint Application Wheel. In addition to combining different modules, nearly all buffers and reagents used within the system are available as standalone items, offering additional customization options for specialized workflows (see Accessory Finder).

In the QIAsprint Blood workflow, samples undergo a straightforward pretreatment and automated purification process. Proteinase K is added to the blood sample and then mixed with Buffer AL to achieve complete enzymatic digestion and efficient lysis (see QIAsprint DNA Blood workflow).

The lysates are then combined with MagG Bead Suspension and isopropanol to enable efficient DNA binding. Wash and the elution plate are prepared according to the protocol and placed into the plate hotels. All plates and hotels are then loaded onto the QIAsprint Connect, and the appropriate protocol is selected. The instrument performs fully automated magnetic-bead–based bind, wash, and elute steps (see QIAsprint DNA Blood workflow).

DNA purified with the QIAsprint DNA Blood workflow is highly suitable for RT-PCR, digital PCR (dPCR) or next-generation sequencing (NGS) as well as long-read sequencing application (depending on disruption and lysis method)