FAQ-2993
What are the recommended lysis times for tissue samples on the QIAsymphony SP?
Lysis time varies depending on the tissue type processed. For recommended lysis times refer to the tissue protocol sheets. If lysis is incomplete after recommended lysis time, as indicated by the presence of insoluble material or highly viscous lysates, lysis time can be prolonged or insoluble material can be removed by centrifugation. Overnight lysis is possible and does not affect the preparation.
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QIAsymphony DSP DNA Kits
QIAsymphony DSP DNA Kits, in combination with the QIAsymphony SP, enable automated total DNA purification from human whole blood, buffy coat, tissues and FFPE tissues, as well as viral DNA from human whole blood for in vitro diagnostic use. Kits are available in mini and midi formats for sample volumes ranging from 200 µl to 1000 µl. For tissue and FFPE tissue protocols, manual sample pretreatment is required. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
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QIAsymphony DNA Kits
QIAsymphony DNA Kits enable automated purification of DNA from a broad range of sample types on the QIAsymphony SP with sample volumes of up to 1 ml. Genomic DNA can be purified from human whole blood, buffy coat, human and animal tissues, formalin-fixed paraffin-embedded tissue (FFPE), cultured cells, and bacterial cultures. The QIAsymphony DNA Mini Kit also enables purification of viral nucleic acids from human whole blood. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
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