| Cell Line Species/Tissue: |
Mouse
/
Embryo |
| Transfection Reagent: |
RNAiFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM: F12, 10ml Transferin, 30nm Natriumslenit |
Percent Serum (%):
|
5% |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
2x10e4 |
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
1µg, 2µg, 3µg |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3µl, 6µl, 6µl |
Complex Incubation on Cells (hrs):
|
4h |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
Not determined |
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| No |
| Notes: |
| |
| References: |
| |
|