Nuclease-Free Water

At 22°C, Nuclease-Free Water has a pH value of between 5.0 and 6.5. It is not possible to determine the pH of highly pure water exactly. Therefore, many publications/industry standards do not provide a pH specification for highly pure water. Highly pure water does not contain enough ions or impurities for an exact pH determination. In general, values between pH 5 and 8 are obtained.

Yes, QIAGEN's Nuclease-Free Water is distilled water that is completely free of substances that may fluoresce.

Nuclease-Free Water has been prepared without the use of chemicals such as DEPC (diethylpyrocarbonate) using an in-house method. The high quality of the water is assured by testing for DNase, RNase, and microbial contamination during the production process.

cDNA generated with the miScript Reverse Transcription Kit can be diluted either with Nuclease-Free Water or TE buffer.