QIAamp Media MDx Kit

For automated purification of DNA from liquid media

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QIAamp Media MDx Kit (12)

Cat. No. / ID: 965752

For 12 x 96 preps: 12 QIAamp 96 Plates, Buffers, Proteinase K, S-Blocks, Disposable Troughs, Racks with Elution Microtubes CL (0.4 ml), Carrier RNA, Top Elute Fluid, Caps, Tape Pad

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The QIAamp Media MDx Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Purification from a variety of liquid transport media
Fully automated procedure with no centrifugation
Purification of viral and other DNA
High-quality DNA with efficient removal of contaminants

Product Details

The QIAamp Media MDx Kit provides a fully automated procedure for purification of DNA from liquid media, such as cervical swab transport media. The fully automated process on the BioRobot MDx workstation, including bar code reading, load check, and complete process documentation, requires only 225 minutes for 96 samples, with no hands-on time.

Performance

Purified nucleic acids are free of proteins, nucleases, and other impurities. Highly reproducible yields enable accurate results in downstream assays (see figure ""). The high-quality nucleic acids are ready to use in downstream assays, such as real-time PCR (see figure "").

See figures

Highly reproducible yields.

Linear yields of high-quality nucleic acids.

Principle

The QIAamp Media MDx Kit uses well-established technology for purification of nucleic acids from liquid media. The kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, for fully automated, simultaneous processing of up to ninety-six 265 µl samples on the BioRobot MDx.

Procedure

Liquid transport media can crosslink cells and form cellular aggregates that are difficult to lyse. Optimized buffers in the QIAamp Media MDx Kit efficiently lyse these samples, stabilize nucleic acids, and enhance selective nucleic acid adsorption to the QIAamp membrane (see flowchart ""). Alcohol is added and lysates are loaded onto the QIAamp 96-well plate. Wash buffers are used to remove impurities, including alcohols and phosphate, and pure, ready-to-use DNA is then eluted in a low-salt buffer. Patent-pending, computer-controlled vacuum technology ensures that volatile contaminants in liquid cytology media, such as alcohols, are efficiently removed.

See figures

QIAamp Media MDx protocol.

Applications

The QIAamp Media MDx Kit can be used for purification of cellular, bacterial, and viral nucleic acids from a variety of sources, including:

Liquid cytology media with alcohol (e.g., PreservCyt and SurePath)
Phosphate-buffered liquid transport media (e.g., M4RT)
Urine
Dried blood spots, blood cards, and swabs

Supporting data and figures

QIAamp Media MDx protocol.

Specifications

Features	Specifications
Applications	PCR, real-time PCR
Elution volume	120 µl
Main sample type	Liquid media
Processing	Automated
Technology	Silica technology
Time per run or per prep	200 minutes (96 samples)
Sample amount	265 µl
Format	96-well plate
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Viral DNA and RNA, bacterial DNA and RNA, cellular DNA and RNA
For automated processing	BioRobot MDx Workstation
Yield	Varies

Resources

For automated purification of nucleic acids from liquid media using the BioRobot MDx workstation

Download Safety Data Sheets for QIAGEN product components.

FAQ

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728