| Cell Line Species/Tissue: |
Mouse
/
Muscle myoblast, fibroblast |
| Transfection Reagent: |
Attractene |
Nucleic Acid:
|
DNA |
Growth Medium:
|
|
Percent Serum (%):
|
10% |
Reporter System:
|
Luciferase, GFP |
Plasmid Purification Method:
|
Sigma |
Plate Format:
|
24-well plate |
Number of Cells:
|
40000 |
Percent Confluence(%):
|
80% |
Amount of Nucleic Acid (µg):
|
4µl |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
1.5µg |
Complex Incubation on Cells (hrs):
|
24h |
Analysis Performed Post-Transfection (hrs):
|
48h |
Transfection Efficiency (%):
|
30% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Achieved 20% transfection efficency with Lipofectamine 2000. |
| References: |
| |