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DNeasy PowerLyzer Microbial Kit (50)
Cat no. / ID. 12255-50
For the bead-based isolation of high-quality DNA from microbial cultures
The DNeasy PowerLyzer Microbial Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Short DNA isolation protocol in just 20 minutes from yeast, fungi and bacterial cultures
- Compatible for use with the PowerLyzer 24 Homogenizer and other bead-based homogenizers
- Ready-to-use, highly pure DNA for downstream applications
Product Details
Isolate high-quality genomic DNA from up to 1.8 ml of microbial culture with the DNeasy PowerLyzer Microbial Kit.
This kit successfully tests a variety of microorganisms, including Gram (+/-) bacteria, yeast and spores, by lysing microorganisms through a combination of heat, detergent and mechanical 0.1 glass bead digestion.
DNeasy PowerLyzer Microbial Kit was formerly sold by MO BIO as PowerLyzer Ultraclean Microbial DNA Isolation Kit.
Supporting data and figures
Figure 1: The right beads for the right machine.
DNeasy PowerLyzer Microbial Kit (glass beads) and the DNeasy UltraClean Microbial Kit (garnet beads) were used to isolate DNA from gram positive E. faecalis bacteria using the PowerLyzer 24 Homogenizer at 2000 RPM for 5 minutes. Ten µl of each eluate was electrophoresed on a 1% agarose gel. Glass beads in the DNeasy PowerLyzer Microbial kit provided higher DNA yield using the PowerLyzer 24 Homogenizer. The DNeasy UltraClean Microbial Kit uses softer, garnet beads that are recommended for bead beating with a Vortex Genie 2.
Specifications
| Features | Specifications |
|---|---|
| Format | Silica Spin Filter Tubes |
| Sample size | 1.8 ml |
| Throughput | 1-50 samples |
| Binding capacity | Up to 20 µg per prep |
| Processing | Bead beating |
| Time per run or per prep | 20 minutes |
| Bead size | 0.1 mm glass |
| Sample types | Processed cultured gram-negative bacteria, gram-positive bacteria, yeast, fungi |
| Storage temperature | Store at room temperature (15-30°C) |
Resources
Safety Data Sheets (1)
Kit Handbooks (2)
Quick-Start Protocols (1)
Certificates of Analysis (1)