PCR 효소 및 키트
특이성과 민감도가 높은 PCR
PCR을 수행할 때 실험의 성공 여부는 처리량, 분석 민감도, 까다로운 템플릿에서도 견고한 반응, 신뢰할 수 있는 데이터 정규화 등 몇 가지 중요한 요소의 영향을 받을 수 있습니다. GC 함량이 높고 템플릿 양이 적으며 반응 조건을 최적화해야 하는 까다로운 템플릿은 추가적인 문제를 야기합니다.
당사의 PCR 시약과 PCR 효소는 실험을 성공으로 이끌고 문제를 극복하는 데 도움이 됩니다. 당사 PCR 키트의 이중 양이온 PCR 완충액은 광범위한 온도 범위에서 높은 프라이머 어닐링 특이성을 유지하므로 각 개별 프라이머–템플릿 시스템에 맞게 PCR을 최적화할 필요가 없습니다.
Product category | Your application goal | Recommended products | When to choose |
|---|---|---|---|
| Hot start PCR |
| For robust, reliable amplification of standard targets | |
| Multiplex PCR | Amplify multiple DNA targets in one reaction | When detecting several targets simultaneously and reducing sample input | |
| One-step RT-PCR | Translate RNA target into DNA in one reaction (RT + PCR) | When starting from RNA and no separate cDNA storage is required | |
| Reverse transcription | Convert RNA into cDNA for downstream applications | When flexible downstream assays or long-term storage of cDNA is needed | |
| High fidelity & long range PCR | High-fidelity amplification for sequencing or cloning | When sequence accuracy or long amplicons (>5–10 kb) are required | |
| PCR enzymes (bulk & OEM) | Custom assay development or OEM supply | For specific or industrial applications and custom formulations |
Find the right end-point PCR product for your workflow
Once you’ve identified the right solution for your application, use the table below to explore detailed specifications of individual PCR kits and enzymes.
It provides key technical parameters such as template type, amplicon length, fidelity, multiplex capability, and performance characteristics — helping you select the most suitable product for your specific workflow.
Product | Template | Amplicon length | Exonuclease activity | Resulting ends | Proof-reading | Multiplex capability | Hot start | Extension time | NGS-ready | GC-Rich or difficult templates |
|---|---|---|---|---|---|---|---|---|---|---|
| Hot Start PCR HotStarTaq DNA Polymerase | DNA | 3–4 kb | Yes 5'→3' | 3' dA overhang | No | No | Yes Chemically-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Hot Start PCR AllTaq PCR Core Kit | DNA | Up to 9 kb | Yes 5'→3' | 3' dA overhang | No | Yes 2 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Multiplex PCR QIAGEN Multiplex PCR Kit | DNA | ∼2–3 kb | Yes 5'→3' | 3' dA overhang | No | Yes Up to 16 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| Multiplex PCR UCP Multiplex PCR Kit | DNA | ∼3–4 kb | Yes 5'→3' | 3' dA overhang | No | Yes Up to 22 targets | Yes Antibody-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| One-step RT-PCR QIAGEN OneStep RT-PCR Kit | RNA | ≤ 2 kb | Yes 5'→3' | 3' dA overhang | No | No | Yes Chemically-mediated | ∼1 kb/min | No | Yes With Q-Solution |
| One-step RT-PCR QIAGEN OneStep Ahead RT-PCR | RNA | ≤ 4 kb | Yes 5'→3' & 3'→5' | Mixture, 3′ dA overhang and blunt ends | Yes | Yes 2 targets | Yes Antibody-mediated | Fast cycling (∼60 min protocol) | No | Yes With Q-Solution |
| Reverse transcription Omniscript RT Kit | RNA | 1–3 kb | N/A | N/A | N/A | No | No | N/A | No | Yes |
| Reverse transcription EnzScript Reverse Transcriptase | RNA | cDNA transcripts >9.4 kb | N/A | N/A | N/A | No | No T° tolerance up to 50°C | N/A | Yes library construction for RNA-Seq transcriptome studies | No |
| High fidelity & long range PCR UltraRun LongRange PCR Kit | DNA | Up to 30 kb | Yes 3'→5' | Blunt ends | Yes [+dU tolerant] | Yes Up to 6 targets | Yes Antibody-mediated | ~1kb / 30s | No | Yes With Q-Solution |
| High fidelity & long range PCR 2x Hi-Fi PCR Master Mix | DNA | Up to 4 kb | Yes 3'→5' | Blunt ends | Yes | Yes library amplification | Yes Antibody-mediated | ~1kb / 30s | Yes NGS library amplification | Yes |
| High fidelity & long range PCR VeraSeq PCR Mix | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes | Yes Can used for higher order amplicon pools for sequencing | No | ~1kb / 15s | No | No |
| High fidelity & long range PCR VeraSeq 2.0 High-Fidelity DNA Polymerase | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes | Yes Can be used for higher order amplicon pools for sequencing | No | ~1kb / 15s | No | Yes with 5X VeraSeq GC buffer |
| High fidelity & long range PCR VeraSeq 2.0 Ultra DNA Polymerase | DNA | Up to 8 kb | Yes 3'→5' | Blunt ends | Yes [+dU tolerant] | Yes Can be used for higher order amplicon pools for sequencing | No | ~1 kb / 15s | No | Yes with 5X VeraSeq GC buffer |
검색 PCR 효소 및 키트
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DNA methylation analysis
26 개의 찾은 제품
QIAGEN OneStep RT-PCR Kit
One-step RT-PCR을 빠르고 성공적으로 할수 있습니다
QIAGEN Multiplex PCR Kit
조건을 잡을 필요없이 쉽고 빠르게 multiplex 분석을 수행 할 수 있습니다
HotStarTaq DNA Polymerase
모든 applications에 최적의 amplication이 가능합니다
HotStarTaq Master Mix Kit
High PCR specificity를 위한 Premixed solution
HotStarTaq Plus DNA Polymerase
별도로 조건을 setup할 필요없이 특이성이 높은 hot-start PCR을 수행할 수 있습니다
Taq DNA Polymerase
For standard and specialized PCR applications
HotStarTaq Plus Master Mix Kit
Fast & specific amplification을 위한 premixed solution
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
Omniscript RT Kit
Reaction당 50 ng부터 2 ug까지의 RNA를 reverse transcription을 할 수 있습니다
Taq PCR Master Mix Kit
편리한 PCR setup을 위한 premixed solution
Taq PCR Core Kit
For standard and specialized PCR applications, includes dNTP mix
QIAGEN Fast Cycling PCR Kit
모든 thermal cycler를 이용하여 빠르고 특이성이 높은 PCR을 수행할 수 있습니다
Sensiscript RT Kit
소량의 RNA (즉, carrier RNA를 포함하여 51 ng RNA 보다 적은 양)로 reverse transcription이 가능합니다
QIAGEN OneStep Ahead RT-PCR Kit
For faster one-step RT-PCR with high sensitivity, specificity and fidelity
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
UCP Multiplex PCR Kit
Ultra-Clean Production master mix for multiplex hot-start PCR and microbiome applications
QIAGEN PCR Cloning Kits
For direct cloning of PCR products generated by Taq DNA polymerases
Type-it Mutation Detect PCR Kit
For accurate and reliable multiplex PCR analysis of mutations
UCP HiFidelity PCR Kit
Ultra-Clean Production master mix for high-fidelity hot-start PCR and microbiome applications
QIAamplifier 96
For fast and high performance end-point PCR experiments in a 96-well format
UltraRun LongRange PCR Kit
For ultrafast, hot-start mediated, long-range PCR
2x HiFi PCR Master Mix
A high efficiency, high-fidelity and low bias PCR master mix for NGS library amplification.
VeraSeq™ 2.0 High-Fidelity DNA Polymerase
Ultrathermostable polymerase for high-fidelity PCR