Isolation of plasmid DNA from Oligotropha carboxidovorans using the QIAGEN® Plasmid Midi Kit - (EN)
The procedure has been used successfully for isolation of the large (128 kb), very-low-copynumber (1-2 copies per cell) plasmid pHCG3 and its derivatives from Oligotropha carboxidovorans. Yield of plasmid DNA was typically 3-6 µg plasmid DNA from 200 ml culture.