Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
Although there may have been sequence updates in Entrez and/or Ensembl which serve as the basis for our QuantiTect Primer Assay design, the assay still matches the amplified region. Therefore, there is no need to design a new assay.
The version number of an assay appears in the assay name as in the following example:
Hs_LTA_2_SG QuantiTect Primer Assay (200)
Hs: human
LTA: lymphotoxin alpha (TNF superfamily, member 1)
2: assay version 2
SG: assay is for SYBR Green based real-time RT-PCR
(200): 200 x 50 µl reactions