Ligation Calculator

This tool determines the mass of insert needed for various inserts:vector molar ratios within the range suitable for typical ligation reactions.

Enter the data of the targets you are interested in and receive sequencing calculations

Required insert DNA mass [g] = vector DNA mass [g] x {insert DNA length / vector DNA length} x desired insert to vector molar ratio

Notes: Desired ratio is one from set {1, 2, 3, 5 ,7}

Insert length should be smaller than vector length

First, the insert and vector DNA is digested using restriction endonucleases to create DNA fragments with sticky (or blunt) ends. Next, the desired DNA fragments are isolated and subsequently joined followed by treatment with DNA ligase. Finally, the resulting intact vector is transformed into competent E. coli cells, and transformants are identified through suitable genetic selection methods.

Want to know more about the step-by-step process of DNA ligation and its typical reaction? Learn more.

Discover enzymes that you can use for ligating DNAs.

Looking for more enzymes?

Discover high-quality products designed for various molecular biology applications.

Explore enzymes

Recommended products

CAT NO./ID L6030-HC-L

T4 DNA Ligase (Rapid)

240,000 U of T4 DNA Ligase (600,000 U/mL) 2x Rapid Ligation Buffer and 10x T4 DNA Ligase Buffer

SEK 8,410.00

CAT NO./ID L6010L

T3 DNA Ligase (900,000 U)

900,000 U of T3 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer

SEK 5,265.00

CAT NO./ID L6020L

T7 DNA Ligase (900,000 U)

900,000 U of T7 DNA Ligase (3,000,000 U/mL) and 2x Rapid Ligation Buffer

SEK 5,265.00

Lorem ipsum dolor sit amet, consectetur adipiscing elit?

Sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur.