Product FAQ

 

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Which sample materials can be tested using the artus Infl./H1 LC/RG RT-PCR Kit?
FAQ ID -2209
Which sample types can be processed using the PAXgene Blood miRNA protocols?
FAQ ID - 3478
Which sample types can be processed using the QIAsymphony PAXgene Blood RNA Protocol?
FAQ ID -2981
Which sample types can be processed using the QIAsymphony Virus Blood 200 protocol?
FAQ ID -2154
Which sample volumes can be used for the QIAsymphony DSP Circulating DNA Kit?
FAQ ID - 3700
Which settings for the filter set Any5Dye have to be adjusted before running the spectral calibration on the Investigator ESSplex Plus?
FAQ ID -2649
Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator ESSplex SE Plus Kit?
FAQ ID -3031
Which settings for the filter set Any5Dye must be adjusted before running the spectral calibration for Investigator IDplex Plus Kit?
FAQ ID -3018
Which siRNA controls are available in FlexiTube format?
FAQ ID -1661
Which software updates are included in a Premium Cover Agreement for my QIAGEN Instrument?
FAQ ID -1336
Which thermal cyclers are validated for use with the Investigator IDplex Kit?
FAQ ID -2403
Which thermal cyclers are validated for use with the Investigator Nonaplex ESS Kit?
FAQ ID -2392
Which transfection reagent is recommended for transfection of the miScript Target Protectors?
FAQ ID -2252
Which tubes are recommended for the QIAsymphony sample rack
FAQ ID - 3653
Which types and amounts of sample material can be processed for RNA extraction on the QIAsymphony SP?
FAQ ID -1937
Which types of collection tubes should preferentially be used for blood samples?
FAQ ID -1496
Which variants of the EasyXtal 15-well tool are available?
FAQ ID -2221
Which version of the Applied Biosystems 7500 Real-Time PCR System software can be used to run the Investigator Quantiplex?
FAQ ID -2569
Which version of the Rotor-Gene Q software can be used to run the Investigator Quantiplex?
FAQ ID -2568
Why and when is it necessary to use carrier DNA/RNA in the extraction procedure?
FAQ ID -1489
Why are most of my samples outside of the cluster in supervised mode using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2205
Why are my qPCR Ct values too high (> 35 or not detectable) in my qRT-PCR assay?
FAQ ID -2685
Why are my qPCR Ct values too low (< 12) in my qRT-PCR Assay?
FAQ ID -2684
Why are my realtime PCR amplification plots hook-shaped?
FAQ ID -587
Why are not all applications/kits/protocol files visible in the run setup (QIAcube Connect App)?
FAQ ID - 1414520
Why are PCR bands relatively weak and "smearing" but the alignment marker bands are sharp when running samples on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1837
Why are samples centrifuged at 4°C after addition of chloroform to the QIAzol lysates in following kits: RNeasy Lipid Tissue Mini/Midi kits, RNeasy Microarray Tissue Mini kit, RNeasy Plus Universal Mini/Midi kit, RNeasy 96 Universal Tissue kit, miRNeasy Mini kit, miRNeasy Micro kit, miRNeasy 96 kit and miRNeasy Serum/Plasma kit?
FAQ ID -2345
Why are samples centrifuged at 4°C after the addition of chloroform when using RNeasy Lipid Tissue Kits?
FAQ ID -533
Why are some of my RNAprotect-stabilized samples frozen atºC while others are not?
ID -758
Why are some of my samples outside of the cluster using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2204
Why are the centrifugation speeds for the QIAamp DNA Mini kit at 6000 x g? Can I spin at full speed?
FAQ ID -474
Why are the denaturation and annealing/extension times for QuantiFast Multiplex RT-PCR Kits much shorter than those for QuantiFast Multiplex PCR Kits?
FAQ ID -2147
Why are the fluorescence intensities of the HSV detection markedly lower than those of the HSV-1 detection using the artus® HSV 1/2 LC PCR Kit?
-2
Why are the QuantiFast Probe Assays located on a single exon, thereby also detecting genomic DNA?
FAQ ID -2365
Why are the QuantiTect and QuantiFast Multiplex PCR Kits limited to triplex real-time PCR on some cyclers?
FAQ ID -715
Why are the RT² qPCR Primer Assays not designed to cross exon-intron junctions or boundaries?
FAQ ID -2710
Why are there no peaks in all channels during a run with the QIAxcel System or QIAxcel Advanced??
FAQ ID -1847
Why are there various different QuantiFast Probe Assays for my gene (previous versions, transcript variants)?
FAQ ID -2366
Why are there various different QuantiTect Primer Assays for my gene (previous versions, transcript variants)?
FAQ ID -1133
Why are two reagents used in the PAXgene Tissue System?
FAQ ID -3022
Why are two reagents used in the PAXgene Tissue System?
FAQ ID - 3603
Why can I not find the Q-Base device in the device list during the first installation?
FAQ ID - 141516
Why can I not find the QuantiTect Primer Assay in GeneGlobe that I had previously ordered from QIAGEN?
FAQ ID -1138
Why can't I find the DNA methylation qPCR Assay for my gene of interest?
FAQ ID -2743
Why can’t I see my QIAcube Connect instrument in the QIAcube Connect App?
FAQ ID - 1414518
Why did the real-time PCR yield Ct values < 12?
FAQ ID -2727
Why did you choose MAPK1 as a positive control in the QIAGEN RNAi Human/Mouse Control Kit?
FAQ ID -604
Why do casework-sample protocols for the QIAsymphony DNA Investigator Kit start with different lysate volumes (200 µl, 500 µl, or 1000 µl)?
FAQ ID -2035
Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?
FAQ ID -1844
Why do I get amplification in a negative control DNA tube using the REPLI-g Kit for WGA?
FAQ ID -675
Why do I get different results for the loci SE33 if I analyze the same sample using the Investigator Nonaplex ESS Kit or Investigator Triplex AFS QS Kit?
FAQ ID -2401
Why do I get genomic DNA contamination in my plasmid prep?
FAQ ID -353
Why do I get smeared PCR products?
FAQ ID -87
Why do I have to add beta-mercaptoethanol (beta-ME) to lysis Buffer RLT of the RNeasy Kits?
FAQ ID -101
Why do I have to use an ACS (Assay Control Set)?
FAQ ID -2933
Why do I have wavy DNA bands on my agarose gel?
FAQ ID -2260
Why do I need normalization using Rotor-Gene ScreenClust HRM Software?
FAQ ID -2198
Why do I need to identify my real-time instrument model when placing my order for RT² qPCR Primer Assays?
FAQ ID -2713
Why do I observe a CT shift for the Internal Control in some samples in comparison to the Ct value for the Internal Control of the dilution series of the Control DNA Z1?
FAQ ID -2575
Why do I observe a CT shift for the internal control in some samples in comparison to the CT value for the internal control of the dilution series of the Male Control DNA M1?
FAQ ID - 3734
Why do I observe a CT shift for the Internal Control in some samples in comparison to the CT value for the Internal Control of the dilution series of the Male Control DNA M1?
FAQ ID - 3745
Why do I see low, poor, or sub-standard amplification efficiency in my qRT-PCR assay?
FAQ ID -2695
Why do I see multiple high-intensity peaks in my qPCR dissociation curve at temperatures less than 70 ºC?
FAQ ID -2690
Why do I sometimes get light blue colonies when using the QIAGEN PCR Cloning Kit?
FAQ ID -603
Why do melting temperatures differ between PCR fragments amplified with QIAGEN's QuantiTect SYBR Green PCR Kits and Roche Kits?
FAQ ID -1084
Why do my qPCR amplification curves or plots decrease in fluorescence intensity after the saturation phase?
FAQ ID -2689
Why do pQE-T7 vectors of the QIAgenes E. coli system have an additional UAG amber stop codon?
FAQ ID -2043
Why do qBiomarker Copy Number PCR Arrays have assays in quadruplicate?
FAQ ID — 3414
Why do QuantiFast SYBR Green PCR Kits require such a high primer concentration?
FAQ ID -1443
Why do replicates in real-time PCR have different plateau heights?
FAQ ID -539
Why do the amplification curves generated with ABI PRISM® 7000 SDS instrument look rather zigzag-shaped when compared to results generated with the ABI PRISM® 7700 SDS instrument?
FAQ ID -1522
Why do the Internal Control templates for extraction (Internal Control DNA or RNA [High conc.]) have a 10x higher concentration than the IC templates provided with the QuantiFast Pathogen PCR +IC Kit and the QuantiFast Pathogen RT-PCR +IC Kit?
FAQ ID -2603
Why do you recommend using Triton X for the purification of 6xHis-tagged protein?
-100
Why does AllPrep DNA/RNA/Protein Mini kit use buffer RLT, but Allprep DNA/RNA Mini and Allprep DNA/RNA Micro kits use buffer RLT Plus? Are these buffers interchangeable?
FAQ ID - 3391
Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?
FAQ ID -1824
Why does my DNA sample float out of the slot when loading it onto an agarose gel?
FAQ ID -205
Why does my isolated RNA have a low OD 260/280 ratio?
FAQ ID -97
Why does my PCR product show up later when comparing the QuantiTect SYBR Green PCR Kits with Roche kits using the same annealing temperature?
FAQ ID -1083
Why does my purified DNA/RNA sometimes have a 260/280 or 260/230 ratio of more than 2? Does that mean the purity is poor?
FAQ ID -3132
Why does my realtime PCR assay quality decrease over time?
FAQ ID -589
Why does TA/UA cloning work with your proofreading HotStar HiFidelity DNA Polymerase?
FAQ ID -1053
Why does the A peak reduction factor have to de set to 0.86 for the codon 61 assay in the therascreen NRAS Pyro Kit?
FAQ ID -2390
Why does the Allprep DNA/RNA 96 Kit use Buffer RLT, whereas the AllPrep DNA/RNA Mini Kit uses Buffer RLT Plus?
FAQ ID -1999
Why does the connection test fail?
FAQ ID - 141522
Why does the copy function not apply relative to migration time and normalized area values after loading the DNA size marker table and adding the alignment marker peaks on the QIAxcel System?
FAQ ID -1832
Why does the DNeasy mericon Food extraction kit use a QIAquick column and not DNeasy column?
FAQ ID -3148
Why does the DNeasy mericon Food Kit use a QIAquick column and Buffer PB rather than a DNeasy column and Buffer AL, which might be expected since the kit isolates genomic DNA?
FAQ ID - 3347
Why does the fluorescence signal drop sharply at the beginning of an artus® PCR run?
FAQ ID -1498
Why does the miScript Target Protector not induce the degradation process?
FAQ ID -2263
Why does the Power LED not function with the QIAxcel or QIAxcel Advanced instrument plugged in and the main power switch turned on?
FAQ ID -1851
Why does the QIAamp DNA Mini Tissue Protocol require both ATL and AL buffer, while the Blood Protocol only uses AL?
FAQ ID -633
Why does the QIAcube sometimes fail to pick up the tips or pick up wrong tips?
FAQ ID -3131
Why does the QuantiTect Primer Assay for my gene of interest have only one version number?
FAQ ID -1134
Why does the SeqTarget Prep Protocol require a 2-step procedure?
FAQ ID -2238
Why does the template for EasyXpress Disulfide Insect Kits need to have a signal sequence (such as the mellitin signal sequence as provided by the EasyXpress Linear Template Fab Kit primers)?
FAQ ID -2967
Why does the upper aqueous phase look pinkish when purifying RNA from fatty tissue?
FAQ ID -3118
Why had my RT² SYBR Green Mastermix been working well in the past, but now does not seem to be?
FAQ ID -2717
Why have the hazard symbols accompanying products changed?
FAQ ID - 3430
Why is 'NO COM' displayed on the QIAxcel Instrument Panel although the power is on and all connections are made to the QIAxcel instrument and computer?
FAQ ID -1848
Why is 18S ribosomal RNA (rRNA) used as a housekeeping gene to normalize sample-to-sample, systematic variation in qPCR assays?
FAQ ID -2675
Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?
FAQ ID -1450
Why is a 2-step (and not a 3-step) cycling protocol recommended for Rotor-Gene SYBR Green Kits?
FAQ ID -2122
Why is a blood collection set required to be used with the PAXgene Blood RNA tube?
FAQ ID - 3459
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
FAQ ID - 3628
Why is an ice-incubation step included during reaction set-up when following the QuantiTect RT-PCR but not the QuantiTect PCR protocol.
FAQ ID -283
Why is carrier RNA used during the isolation of gDNA from microdissected samples with the QIAamp DNA Micro Kit?
FAQ ID -473
Why is CoralLoad included in the Type-it Mutation Detect, but not in the Type-it Microsatellite PCR Kit?
FAQ ID -2068
Why is DTT used only for semen samples?
FAQ ID - 143761
Why is EvaGreen instead of SYBR Green used as fluorescent dye in the Type-it HRM PCR Kit?
FAQ ID -2196
Why is it not recommended to stabilize cells with RNAprotect?
FAQ ID -941
Why is it recommended to add 1 mM IPTG for optimal protein yields using the EasyXpress Protein Synthesis Kits?
FAQ ID -860
Why is maximum amplicon size for the Type-it Microsatellite PCR Kit limited to 500 bp?
FAQ ID -2060
Why is my 260/280 ratio low after using the DNeasy mericon Food Kit?
FAQ ID - 3349
Why is my EpiTect Methyl qPCR Assay "failed" as indicated in the QC page of the data analysis Excel file?
FAQ ID -2734
Why is my no template control (NTC) real-time Ct value < 35 cycles in my qPCR Assay?
FAQ ID -2686
Why is my plasmid DNA yield low?
FAQ ID -768
Why is special Type-it Fast SNP Probe PCR chemistry required for TaqMan® SNP Genotyping?
FAQ ID -2057
Why is the activation time for HotStarTaq Plus Polymerase in the QuantiFast SYBR Green Kits different from that for QuantiFast Probe Kits?
FAQ ID -1449
Why is the alignment marker for the 1.8 kb fragment not visible when using the 25 bp/1.8 kb DNA Size Marker together with the 15 bp/400 bp marker on the QIAxcel System and QIAxcel Advanced?
FAQ ID -1835
Why is the copy number call lower than expected?
FAQ ID — 3413
Why is the final heat denaturation step so important with the QIAsymphony PAXgene Blood RNA protocol?
FAQ ID -2987
Why is the fluorescence signal of the Internal Control on my Rotor-Gene Q very low?
FAQ ID -2574
Why is the IC not detectable although the analytical PCR is negative?
FAQ ID -1506
Why is the IC not detectable although the analytical PCR is positive?
FAQ ID -1505
Why is the initial activation step different for Rotor-Gene Probe, SYBR Green and Multiplex Kits?
FAQ ID -2118
Why is the initial fluorescence signal too high when using artus PCR assays on the Rotor-Gene cycler?
FAQ ID -1497
Why is the magnesium (Mg2+) solution supplied in a separate tube in the artus® Parvo B19 PCR Kits?
FAQ ID -1533
Why is the pQE DNA provided in QIAexpress Kits blue in color?
FAQ ID -487
Why is the QIAamp Viral RNA Mini Kit (50) currently unavailable?
FAQ ID -147395
Why is the QIAcube Connect App reacting so slowly?
FAQ ID - 141519
Why is the qRT-PCR reproducibility so critical when detecting gene expression knock down in an RNAi experiment?
FAQ ID -2774
Why is the QuantiFast denaturation step different for PCR and RT-PCR runs in the two-step protocol for the ABI 7500 and other cyclers?
FAQ ID -1442
Why is the reaction volume for QuantiFast PCR Kits lower than that for QuantiTect PCR Kits?
FAQ ID -1447
Why is the RT step with the QuantiFast RT Kits much shorter compared to QuantiTect RT Kits?
FAQ ID -1451
Why is the storage time for QuantiFast PCR Kits shorter than that for QuantiTect PCR Kits?
FAQ ID -1446
Why is the Type-it Mutation Detect PCR Kit recommended for preamplification of SNPs?
FAQ ID -2067
Why is there an asterisk for my miScript Primer Assays?
FAQ ID -2176
Why is there DNA in the no-template (negative) control when using the QuantiTect Whole Transcriptome Kit?
FAQ ID -1620
Why is there DNA in the no-template control reaction when using the standard REPLI-g procedure, but not when using the UltraFast procedure?
FAQ ID -1327
Why is there more than one QIAgene for my gene of interest?
FAQ ID -2044
Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732
Why is there no male DNA obtained from sexual assault samples?
FAQ ID - 143767
Why is there no QuantiTect Primer Assay for my gene of interest?
FAQ ID -1140
Why is there no vacuum-only protocol for the RNeasy Plus 96 Kit?
FAQ ID -1993
Why is there RNA in the DNA eluate when using the AllPrep DNA Mini Spin Column?
FAQ ID -1044
Why might Affymetrix GeneChip Mapping assays interfere with the REPLI-g FFPE kit?
FAQ ID -1755
Why might my gene of interest drop out during WGA if it is not near a telomere or centromere?
FAQ ID -703
Why should DNA or cDNA targets be less than 250 bp long for real-time PCR?
FAQ ID-751
Why should I use RT² SYBR Green Mastermix with RT² qPCR Primer Assays?
FAQ ID -2706
Why should I use the EpiTect Plus FFPE Bisulfite Kit instead of isolating FFPE DNA first and then doing the bisulfite conversion?
FAQ ID -2412
Why should I use the RNeasy Microarray Tissue Mini Kit when purifying RNA for microarray analysis?
FAQ ID -2188
Why should I work with diluted or undiluted screens?
FAQ ID -2419
Why would clumps occur following the addition of Buffer P2 when using LyseBlue Reagent in a plasmid preparation?
FAQ ID -862
Will acetone precipitation recommended in the Qproteome Protocols denature Protein?
FAQ ID -807
Will biotin detection assays be efficient when doing in vitro translation from E. coli lysates with the EasyXpress Site-Specific Biotin Kit?
FAQ ID -878
Will DNA from chromosomes collected in TE with a drop of mineral oil work with REPLI-g amplification?
FAQ ID -664
Will dUTP in a PCR reaction affect pyrosequencing?
FAQ ID -2843
Will integral, peripheral as well as lipid-anchored plasma membrane proteins be isolated with the Qproteome Plasma Membrane Protein Kit?
FAQ ID -1199
Will mitochondrial DNA also be isolated using the DNA tissue protocols and QIAsymphony DSP DNA Mini Kit on the QIAsymphony SP?
FAQ ID -2991
Will mitochondrial DNA also be isolated using the DNA Tissue protocols on the QIAsymphony SP?
FAQ ID -1921
Will pancreas tissue swim on top of RNAprotect?
FAQ ID -536
Will pipetting error affect the EpiTect Methyl qPCR Array results?
FAQ ID -2741
Will pipetting error affect the miRNA qPCR Assay results?
FAQ ID -2728
Will proteins eluted in the Qproteome Plasma Membrane Protein protocol be modified, e.g. with the ligand?
FAQ ID -1195
Will qBiomarker Copy Number Assays work with heterogeneous samples like mixtures of tumor and normal tissue?
FAQ ID — 3419
Will QIAseq FastSelect work in other species?
FAQ ID -147911
Will QuantiTect Primer Assays work at an annealing temperature of 60ºC with QuantiFast SYBR Green PCR and RT-PCR Kits?
FAQ ID -1553
Will QuantiTect Primer Assays work with Rotor-Gene SYBR Green Kits using an annealing step at 60ºC?
FAQ ID -2124
Will REPLI-g work at high temperatures?
FAQ ID -656
Will Rotor-Gene Kits also work on the Rotor-Gene 6000 and 3000 cyclers?
FAQ ID -2121
Will site-specific biotin labeling with the EasyXpress Site-Specific Biotin kit alter the structure and function of my protein?
FAQ ID -877
Will TA cloning work with REPLI-g WGA product?
FAQ ID -672
Will the "classic" RNeasy Mini Kit be discontinued after the launch of the Allprep DNA/RNA Mini Kit?
FAQ ID -1054
Will the 2x QIAGEN Multiplex PCR Master Mix freeze at -20°C?
FAQ ID - 525
Will the FastLane Cell cDNA Kit work with suspension cells?
FAQ ID -801
Will the ligand in the Qproteome Plasma Membrane Protein protocol be specific for plasma membrane exclusively?
FAQ ID -1198
Will the optimized DNA sequence for QIAgenes be provided?
FAQ ID -2045
Will the PhosphoSerine antibody recognize Serine residues if the neighboring residues are Leucine or Isoleucine?
FAQ ID -644
Will the primer sequence for the PyroMark CpG Assay be provided?
FAQ ID -2823
Will the QIAquick PCR Purification Kit remove sufficient SYBR Green from real-time PCR reactions to allow sequencing?
FAQ ID -637
Will the QIAxcel System run using a green Data Review Software Key?
FAQ ID -1853
Will the Qproteome Plasma Membrane Protein Kit work with starting materials other than adherent mammalian cells?
FAQ ID -1202
Will the random hexamers in the REPLI-g reaction interfere with downstream analysis?
FAQ ID -713
Will the REPLI-g UltraFast Mini Kit reaction always yield 10 µg DNA?
FAQ ID -1328
Will the Reverse transcription control on the RT2 profiler PCR array work on any cDNA library?
FAQ ID - 3534
Will the sequence of the QuantiFast Probe Assays be provided?
FAQ ID -2369
Will the sequences of the QuantiTect Primer Assays be provided?
FAQ ID -804
Will Uracil-N-Glycosylase (UNG) completely remove contaminating amplicons when using QuantiTect +UNG Kits?
FAQ ID -2139
With LyseBlue reagent for lysis control, can I now process more bacterial culture and overload the columns?
FAQ ID -864
With which platform can I perform my sequencing?
FAQ ID - 143072
With which read length should the EpiTect Hi-C NGS libraries be sequenced
FAQ ID - 143073
Would co-transfection with the plasmid be expected to increase/decrease the transfection efficiency significantly?
FAQ ID -3136
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