| Cell Line Species/Tissue: |
hs
/
|
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
|
Percent Serum (%):
|
6 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
48-well plate |
Number of Cells:
|
16000 |
Percent Confluence(%):
|
35 % |
Amount of Nucleic Acid (µg):
|
20nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
1 |
Complex Incubation on Cells (hrs):
|
24 hrs |
Analysis Performed Post-Transfection (hrs):
|
72 hrs |
Transfection Efficiency (%):
|
No |
| Knockdown Efficiency (%): |
Very good |
| |
| Any modifications to the protocol?:
|
| None |
| Notes: |
| Revrese Transfection Protocol |
| References: |
| |