连接计算器

此工具确定了在适用于典型连接反应的范围内的各种插入片段:载体摩尔比率所需的插入片段质量。

输入您感兴趣的靶标的数据并接收测序计算结果

所需的 DNA 插入片段质量 [g] = 载体 DNA 质量 [g] x {DNA 插入片段长度 / 载体 DNA 长度} x 期望的插入片段与载体摩尔比率

提示：期望的比率为集合 {1, 2, 3, 5 ,7} 中的一个

插入片段长度应小于载体长度

首先，使用限制性内切酶消化插入片段和载体 DNA，以产生具有黏端或平端的 DNA 片段。接下来，提取期望的 DNA 片段，随后连接，然后用 DNA 连接酶进行处理。最后，将得到的完整载体转化到感受态 E. coli 细胞中，并通过合适的遗传选择方法鉴定转化体。

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了解可用于连接 DNA 的酶产品。

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