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AllPrep DNA/mRNA Nano (12)
Kat.-Nr. / ID. 80272
For 12 preps: Oligo-dT magnetic beads, DNA-isolation beads, collection tubes, RNase-free water and buffers
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AllPrep DNA/mRNA Nano
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AllPrep DNA/mRNA Nano Kit ist für molekularbiologische Anwendungen vorgesehen. Dieses Produkt ist nicht für die Diagnose, Prävention oder Behandlung einer Krankheit bestimmt.
✓ Automatische Verarbeitung von Online-Bestellungen 24/7
✓ Sachkundiger und professioneller technischer und Produkt-Support
✓ Schnelle und zuverlässige (Nach-)Bestellung
Eigenschaften
- Simultaneous gDNA and mRNA isolation
- Maximal yields from minimal cell quantities
- High-molecular-weight, sequencing-ready gDNA
- Messenger RNA suitable for single-cell RNA-seq application
- Magnetic-bead-based system
Angaben zum Produkt
Collect genetic and transcriptomic information from CTCs and rare cells, with minimal cell quantities, by isolating gDNA and mRNA from the same sample, at the same time.
The AllPrep DNA/mRNA Nano Kit uses a two-step magnetic-bead-based method that lets you maximize mRNA capture and isolate high-molecular-weight DNA for multiomics studies.
Leistung
The AllPrep DNA/mRNA Nano Kit purifies high-quality gDNA and mRNA from the same cell (see figure “ Simultaneous mRNA and gDNA purification”) or low-biomass input samples (see figures “ Messenger RNA for single-cell RNA-seq applications”, “ Sequencing-ready gDNA from low cell numbers”, and “ Minimal amount of cells required”).
The kit’s simple and convenient procedure does not require the use of toxic substances such as phenol (see figure “ Magnetic-bead-based system”).
Genomic DNA purified with the AllPrep mRNA/DNA Nano Kit procedure has an average length of 60–100 kb, depending on homogenization conditions (see figure “ High-molecular-weight gDNA”). Compared to products from other suppliers, the AllPrep mRNA/DNA Nano Kit provides nucleic acids of significantly higher yields (see figure “ Maximal DNA and mRNA yields”).
Prinzip
Unlike other procedures where the biological sample or purified total nucleic acids are divided into two before being processed separately, the AllPrep DNA/mRNA Nano Kit purifies gDNA and mRNA simultaneously from the same precious sample. It also allows the parallel processing of multiple samples in less than 75 minutes.
The kit is compatible with small amounts of low-biomass samples such as CTCs, rare cells and low numbers of cells of animal or human origin.
The use of toxic substances such as phenol and/or chloroform, and tedious methods such as alcohol precipitation, are replaced by safe and simple magnetic-bead-based workflows using a magnetic rack such as the AdnaMag-S.
Verfahren
The AllPrep mRNA/DNA Nano Kit’s magnetic-bead-based system purifies mRNA using oligo-dT magnetic beads, while gDNA is purified using DNA-affinity beads.
Anwendungen
Genomic DNA purified with the AllPrep DNA/mRNA procedure has a high molecular weight of up to 100 kb. This purified DNA is suitable for any downstream application, such as PCR, multiplex PCR, dPCR, pyrosequencing or NGS.
The purified mRNA is of high quality and can be used in any application, such as RT-PCR, real-time RT-PCR, or cDNA generation, including the AdnaTest procedure for molecular characterization of CTCs.
Ergänzende Daten und Abbildungen
Simultaneous mRNA and gDNA purification.
Six samples containing AllPrep DNA/mRNA Nano lysis buffer were each spiked with 5 MCF-7 cells and 5 LNCaP95 cells. Messenger RNA and genomic DNA were isolated using the AllPrep DNA/mRNA Nano Kit. Messenger RNA was analyzed using the AdnaTest BreastCancerDetect Kit (GA733-2 expression profile), and matched genomic DNA yield (H91; human target, small autosomal, 91 bp) was determined using the Investigator Quantiplex Pro RGQ Kit.
A range of 7.9–11.9 ng/µl (median: 10 ng/µl) of mRNA was isolated, and gDNA was detected within 28.3–29.6 cycles (median Cq: 28.8).
Spezifikationen
| Eigenschaften | Spezifikationen |
|---|---|
| Applications | Transcriptome and genome profiling, real-time PCR, cDNA synthesis, NGS, RNA seq |
| Elution volume | 25 µl |
| Format | Magnetic-bead-based system |
| Main sample type | Low-biomass samples (rare cells, CTCs, fetal cells, stem cells, T-cells) |
| Processing | Manual (magnetic rack) |
| Product use | Purification of full-length messenger RNA and high-molecular-weight genomic DNA |
| Sample amount | Down to 1 cell |
| Technology | Magnetic beads |
| Time per run or per prep | 75 min per prep |
Ressourcen
Safety Data Sheets (1)
Kit Handbooks (1)
Quick-Start Protocols (1)
Broschüren und Leitfäden (1)
FAQ
Can miRNA and other RNAs smaller than 200 nucleotides be isolated with the AllPrep DNA/mRNA Nano Kit?
What is the minimum amount of starting material that can be used with the DNA/mRNA Nano Kit?