PAXgene Bone Marrow RNA Tubes
For collection of bone marrow samples and stabilization/purification of intracellular RNA
My QIAGEN
The place to manage all of your QIAGEN purchases, quotes, instruments, kits, subscriptions and service requests.
PAXgene Bone Marrow RNA Tubes (50) icon_0368_ls_gen_eco_friendly-s
Cat no. / ID. 764114
50 Bone Marrow Collection Tubes. To be used in conjunction with the PAXgene Bone Marrow RNA Kit
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
My QIAGEN
The place to manage all of your QIAGEN purchases, quotes, instruments, kits, subscriptions and service requests.
Features
- To be used in conjuction with the PAXgene Bone Marrow RNA Kit
- Immediate stabilization of intracellular RNA upon introducing the sample into the tube
- Rapid purification of RNA from heterogeneous bone marrow samples using the PAXgene Bone Marrow RNA Kit
- RNA is stable for days at room temperature or refrigerated
- Long-term sample storage at –20°C or –70°C
Product Details
The PAXgene Bone Marrow RNA System consists of PAXgene Bone Marrow RNA Tubes, for stabilization, storage, and transportation of bone marrow, and the PAXgene Bone Marrow RNA Kit, for silica-membrane-based RNA isolation and purification in a spin-column format. Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is immediately transferred into a PAXgene Bone Marrow RNA Tube, which stabilizes the RNA in the sample for storage at room temperature, refrigerated, or frozen. RNA can be purified days later using the PAXgene Bone Marrow RNA Kit or archived for several months at –20°C or –70°C.
Performance
The PAXgene Bone Marrow RNA Kit enables efficient isolation and purification of intracellular RNA from heterogeneous human whole bone marrow samples (see figure " RNA yields from bone marrow samples"), including an optimized procedure for cell-rich, viscous samples. RNA can be purified days after collection, and intact, high-quality RNA can be purified using the PAXgene Bone Marrow RNA Kit after samples are stored at room temperature, refrigerated, or frozen (see figures " RNA stability at room temperature", " RNA stabilized at 2–8°C", and " High RNA integrity after freeze–thaw cycles"). RNA Tubes protect RNA molecules from degradation by RNases and enable accurate analysis by minimizing ex vivo changes in gene expression (see figure " Stability of 5 transcripts at room temperature"). An integrated DNase treatment ensures that genomic DNA is minimized. In-house and external studies showed that genomic DNA was <0.85% in 95% of the samples tested (n = 157). A260/A280 values are typically between 1.8 and 2.2, indicating the high purity of the RNA (see figure " Storage under different conditions").
Principle
Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at room temperature, making reliable studies of gene expression impossible. PAXgene Bone Marrow RNA Tubes protect RNA molecules from degradation and immediately stabilize the RNA in the sample for storage at room temperature, refrigerated, or frozen.
Procedure
Bone marrow samples are collected using standard medical practices, with commonly used anticoagulants. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube. The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. The PAXgene Bone Marrow RNA Kit provides an easy and rapid procedure for isolation and purification of intracellular RNA from human whole bone marrow samples. The kit uses silica-membrane technology in a spin-column format for efficient purification of intact, high-quality RNA.
Applications
RNA is stabilized in PAXgene Bone Marrow RNA Tubes and can be used in a range of downstream applications, including:
- cDNA synthesis
- Gene expression arrays
- End-point RT-PCR
- Quantitative RT-PCR, including TaqMan technology
Supporting data and figures
Stability of 5 transcripts at room temperature.
The changes in mRNA levels (ΔCT) from day 0 to day 2 for the samples in figure "RNA stability at room temperature" were calculated for each transcript quantified. The minimal changes and small standard deviation for samples stabilized in PAXgene Bone Marrow RNA Tubes contrasts with the significant changes for samples stored in EDTA tubes.
Specifications
| Features | Specifications |
|---|---|
| Tube size | 16 x 125 mm |
| Label type | Paper |
| Draw volume | 2 ml |
| Quantity | 50/box |
| Additive/concentration | Additive 11.1 ml |
| Closure type/color | BD Hemogard/Red |
| Stabilization | up to 3 days at room temperature (15–25°C); up to 5 days at 2–8°C; long-term at –20°C or –70°C |
Resources
Brochures & Guides (2)
Safety Data Sheets (1)
Scientific Posters (2)
Gene Expression Analysis (1)
Kit Handbooks (2)
Certificates of Analysis (1)