How can I improve my RNA yield from liver sample when using RNeasy Kits?
Sometimes, liver causes difficulties when used as starting material for the RNeasy Mini/Midi/Maxi Protocol for isolation of total RNA from Animal Tissue. This can be resolved by adding 1 volume of 50% ethanol to the cleared RLT lysate before applying it to the RNeasy column, instead of the 70% ethanol specified in the RNeasy Mini Kit Handbook and RNeasy Midi/Maxi Kit Handbook.