Why do DNA samples appear in only a few channels when the alignment marker is working using the QIAxcel System or QIAxcel Advanced?

The working alignment marker is an indication that the cartridge channel is working well. DNA samples that do not appear in all channels of the QIAxcel System or QIAxcel Advanced may be caused by:

1. Low sample volume (< 10 ul). The minimum required sample volume is 10 ul. 
2. Trapped air bubble in the well. The air bubble must be removed. 
3. High salt concentration in the sample. Dilute the sample with the QX DNA or RNA dilution buffer and rerun the sample.