FAQsHomeFAQs3274 - Can I do singleplex PCR with the...3274 - Can I do singleplex PCR with the QuantiFast Probe Assays?Yes, for 2-step RT-PCR, the QuantiFast Probe PCR Kit can be used, for 1-step RT-PCR, the QuantiFast Probe RT-PCR Plus Kit is combined with the assays.