What kind of control samples should I use for the particular iPSC-qBiomarker Arrays I have ordered?

It is recommended to always include negative and positive control samples to facilitate data interpretation. In general, a negative control sample should be your starting cell line and a positive control sample should be a validated cell line that you wish your cells to be similar to.

For qBiomarker iPSC colony screening PCR Arrays, (catalog # IPHS-001) the negative control sample should be the iPSC parental cell line (such as fibroblasts) and the positive control sample should be an established embryonic stem cell line (such as H9) or a validated iPSC cell line.

For qBiomarker Yamanaka factors expression PCR Arrays (catalog # IPHS-002) the negative control sample should be the parental cell line (such as fibroblasts) and the positive control sample should be an established embryonic stem cell line (such as H9) or a validated iPSC cell line. Use of a third control sample, the day 1 transfected cells (Parental cell line transfected by expression vectors for the four transcription factors in the Yamanaka cocktail for 24 hours) is strongly recommended. This third control sample will monitor the expression of your transgenes.

For qBiomarker Thomson factors expression PCR Arrays, (catalog # IPHS-003) the negative control sample should be the parental cell line (such as fibroblasts) and the positive control sample should be an established embryonic stem cell line (such as H9) or a validated iPSC cell line. Use of a third control sample, the day 1 transfected cells (Parental cell line transfected by expression vectors for the four transcription factors in the Thomson cocktail for 24 hours) is strongly recommended. This third control sample will monitor the expression of your transgenes.

For qBiomarker iPSC pluripotency validation PCR Arrays, (catalog # IPHS-100) the negative control sample should be the parental cell line (such as fibroblasts) and the positive control sample should be an established embryonic stem cell line (such as H9) or a validated iPSC cell line.

For qBiomarker Embryoid body differentiation validation PCR Arrays, (catalog # IPHS-101) the negative control sample should be the starting iPSC or embryonic stem cell line, and the positive control sample should be a validated embryoid body sample.

For qBiomarker Cardiomyocyte differentiation PCR arrays, (catalog # IPHS-102 the negative control sample should be the starting iPSC or embryonic stem cell line, and the positive control sample should be validated cardiomyocyte cells.

For qBiomarker Neuronal Differentiation PCR Arrays, (catalog # IPHS-103) the negative control sample should be the starting iPSC or embryonic stem cell line, and the positive control sample should be validated neuronal cells.

For qBiomarker Reprogramming factors expression PCR Arrays, (catalog # IPHS-104) the negative control sample should be the parental cell line (such as fibroblasts) and the positive control sample should be an established embryonic stem cell line (such as H9) or a validated iPSC cell line. Use of a third control sample, the day 1 transfected cells (Parental cell line transfected by expression vectors for the transcription factors in the reprogramming cocktail for 24 hours) is strongly recommended. This third control sample will monitor the expression of your transgenes.