What is the average RNA yield from PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue?

RNA yield depends on several parameters, such as tissue type, time from resection until fixation, fixation time (ideally 2 to 4 hours), processing protocol used and age and storage conditions of the PFPE block.

In a study with PFPE tissue sections (area: 100 mm²;  thickness: 10 µm) median RNA yield from rat liver was 4.2 µg (n=58), from kidney 2.2 µg (n=58), from spleen 4.7 µg (n=58), from intestine 4.7 µg (n=58) and from lung 0.9 µg (n=58) (see Technical Note "Yield, purity, and integrity of RNA purified from PAXgene Tissue fixed, paraffin-embedded (PFPE) rat tissue" under the Product Resources tab).