How can QIAGEN Protease and Proteinase K be inactivated?
QIAGEN Protease is inactivated by incubation at 70°C for 15 minutes.
To our knowledge, Proteinase K cannot be completely heat-inactivated. Even when incubating at 95°C for 10 minutes, some enzymatic activity remains. This will not negatively affect the QIAamp Procedure, since the enzyme will be efficiently removed by the wash steps in the protocols.