What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?

Researchers generally use small nucleolar RNAs (snoRNAs) or 5S RNA as the normalizer for miRNA.

To normalize miRNA real-time PCR data obtained with the miScript System, QIAGEN offers primer assays for 15 different snoRNAs and for 5S RNA. Any of these can be used for normalization.

 

icon_0011_idea-s

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.