What controls should be used for normalizing miRNA real-time PCR data obtained with the miScript System?
Researchers generally use small nucleolar RNAs (snoRNAs) or 5S RNA as the normalizer for miRNA.
To normalize miRNA real-time PCR data obtained with the miScript System, QIAGEN offers primer assays for 15 different snoRNAs and for 5S RNA. Any of these can be used for normalization.