| Cell Line Species/Tissue: |
human
/
hybrid of HUVECs and A549 |
| Transfection Reagent: |
SuperFect |
Nucleic Acid:
|
DNA |
Growth Medium:
|
DMEM |
Percent Serum (%):
|
|
Reporter System:
|
GFP |
Plasmid Purification Method:
|
2x CsCl |
Plate Format:
|
|
Number of Cells:
|
approx. 1x106 |
Percent Confluence(%):
|
50-60 |
Amount of Nucleic Acid (µg):
|
2.5 |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
15 |
Complex Incubation on Cells (hrs):
|
2 |
Analysis Performed Post-Transfection (hrs):
|
48 |
Transfection Efficiency (%):
|
20-30% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| |
| References: |
| |