Transfection Cell Database - Detailed View


Cell Line: DU145

Cell Line Species/Tissue: Human /  
Transfection Reagent: HiPerFect 
Nucleic Acid:
siRNA (dsRNA) 
Growth Medium:
MEMalpha 
Percent Serum (%):
10% FCS 
Reporter System:
 
Plasmid Purification Method:
 
Plate Format:
12-well 
Number of Cells:
20.000 cells/well 
Percent Confluence(%):
 
Amount of Nucleic Acid (µg):
100nM 
Amount of Enhancer (µl):
 
Amount of Reagent (µl):
6µl 
Complex Incubation on Cells (hrs):
72 h 
Analysis Performed Post-Transfection (hrs):
72 h 
Transfection Efficiency (%):
 
Knockdown Efficiency (%): 100%  
 
Any modifications to the protocol?:
500µl Transfection medium, Medium change after 6h  
Notes:
 
References: