| Cell Line Species/Tissue: |
Monkey
/
Kidney |
| Transfection Reagent: |
Effectene |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
DMEM, 100 U/ml penicillin, 100 µg/ml streptomycin |
Percent Serum (%):
|
10% FCS |
Reporter System:
|
eGFP |
Plasmid Purification Method:
|
|
Plate Format:
|
24-well |
Number of Cells:
|
3x10e4 |
Percent Confluence(%):
|
|
Amount of Nucleic Acid (µg):
|
0.2 µg |
Amount of Enhancer (µl):
|
1.6 µl |
Amount of Reagent (µl):
|
5 µl |
Complex Incubation on Cells (hrs):
|
24 hrs |
Analysis Performed Post-Transfection (hrs):
|
24 - 48 hrshrs |
Transfection Efficiency (%):
|
70% |
| |
| Any modifications to the protocol?:
|
| |
| Notes: |
| Each component in the cotransfection experiment was represented by 0.06 µg of DNA, and
the total amount of DNA was supplemented to 0.2 µg/well with empty pcDNA3.1 vector DNA if necessary. |
| References: |
| Signal transduction of somatostatin in human B lymphoblasts
DIETER ROSSKOPF, MARKUS SCHU¨ RKS, IRIS MANTHEY, MARKUS JOISTEN, STEFAN BUSCH, AND WINFRIED SIFFERT
Am J Physiol Cell Physiol 284: C179–C190, 2003. |