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EZ1 ccfDNA Midi Kit (48)
Cat. No. / ID. 954154
For 48 preps (4 or 5 ml sample input volume each): 48 reagent cartridges (EZ1 ccfDNA), QIAGEN Proteinase K, Magnetic Bead Suspension, Buffers, Bead Elution Tubes, Disposable Tip Holders, Disposable Filter-Tips, Elution Tubes (1.5 ml)
KitCard
EZ1 ccfDNA Kit
EZ1 Advanced XL ccfDNA Card
Sample volume
5–10 ml
1–4 ml
EZ1 ccfDNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease..
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Efficient recovery of fragmented DNA
- Scalable sample input volume of 1–10 ml
- No organic extraction or ethanol precipitation
- Removal of contaminants and inhibitors
- Semi-automated, convenient and streamlined protocol
Product Details
EZ1 ccfDNA Kits provide semi-automated concentration and purification of circulating cell-free DNA from plasma or serum using the EZ1 Advanced XL Instrument.
Performance
EZ1 ccfDNA Kits enable efficient purification of circulating DNAs from human plasma or serum. Upfront manual pre-concentration of ccfDNA on magnetic beads allows flexible and scalable handling of volumes from 1 to 10 ml. Subsequent ccfDNA cleanup on the EZ1 Advanced XL instrument provides convenient automated bead-based purification of up to 14 samples in parallel.
Free-circulating DNA is ready for use in downstream reactions or storage at –30 to –15°C. Purified nucleic acids are free of proteins, nucleases and other impurities.
The EZ1 ccfDNA Mini Kit has been optimized for sample volumes from 1 to 4 ml while the EZ1 ccfDNA Midi Kit has been optimized for sample volumes from 4 to 10 ml. Yield depends on the sample volume and the concentration of circulating nucleic acids in the sample (typically 1–100 ng/ml in plasma).
Principle
Free-circulating DNA, such as tumor-specific extracellular DNA fragments in the blood or fetal DNA in maternal blood, are present in serum or plasma usually as short fragments of <1000 bp.
EZ1 ccfDNA Kits enable efficient purification of these circulating DNAs from human plasma or serum.
EZ1 ccfDNA Kits enable efficient purification of these circulating DNAs from human plasma or serum.
Procedure
EZ1 ccfDNA Kits use a fast procedure involving manually carried out pre-concentration of circulating nucleic acids onto magnetic beads and cleanup of the resulting pre-eluate on the EZ1 Advanced XL Instrument, all in a quick and convenient automated procedure.
The magnetic-particle technology combines the speed and efficiency of silica-based nucleic acid purification with the convenient handling of magnetic particles. The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples.
The simple procedure, which is suited for simultaneous processing of multiple samples, provides pure nucleic acids in less than 1 hour for 14 samples.
Applications
EZ1 ccfDNA Kits provide semi-automated concentration and purification of circulating cell-free DNA from plasma or serum using the EZ1 Advanced XL Instrument.
Supporting data and figures
Efficient purification of ccfDNA
Total yield from 1 ml of plasma is shown using the EZ1 ccfDNA Mini Kit or a plasma kit from Supplier P.
Resources
Quick-Start Protocols (2)
Safety Data Sheets (3)
Kit Handbooks (1)
Certificates of Analysis (1)