FlexiGene DNA Kit

For scalable isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

FlexiGene DNA Kit (250)

Cat. No. / ID: 51206

For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation

$439.00

Quantity

The FlexiGene DNA Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

Easy handling, no risk of sample mix-up
Scalable purification from 0.1–20 ml whole blood
Direct purification from whole blood, buffy coat, and cultured cells
Fast procedure
No organic extraction

Product Details

The FlexiGene system uses simple centrifugation to isolate highly pure DNA from whole blood, buffy coat, and cultured cells. The FlexiGene DNA Kit contains buffers and QIAGEN Protease for direct, simple purification in a single tube. For automated solutions using FlexiGene reagents, contact Autogen, Inc.

Performance

DNA purified using the FlexiGene system is sized up to 150 kb (see figure " High-quality genomic DNA"). It performs well in amplification and Southern blotting experiments, and is also suitable for archiving (see figure " Efficient downstream PCR analysis").

DNA is purified from 0.1–20 ml (blood), 0.1–2 ml (buffy coat), or 1–2 x 10⁶ cells (cultured cells) in a single tube, processed by centrifugation. Twelve samples can be processed in 1 hour. Depending on sample size, the rehydration volume is 0.2–1 ml.

DNA yields purified from different sample types using the FlexiGene procedure
Sample	Volume	DNA yield (µg)
Blood*	0.3 ml	11–14
Blood*	2.0 ml	75–90
Blood*	10.0 ml	380–460
Buffy coat	0.5 ml	110–130
HeLa cells	10⁶ cells	25–35

* Whole blood with normal white blood cell counts (7x10⁶ cells/ml blood).

See figures

High-quality genomic DNA.

Efficient downstream PCR analysis.

Principle

The FlexiGene DNA system enables isolation of genomic DNA from variable amounts of whole blood, buffy coat, and cultured cells. The simple purification procedure is performed in a single tube, which reduces costs and simplifies handling. The purified DNA performs well in sensitive downstream applications.

Procedure

The first step is addition of lysis buffer to the samples (see flowchart ""). Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC. High DNA yields are obtained from whole blood, buffy coat, and cultured cells (see table "DNA yields purified from different sample types using the FlexiGene procedure").

See figures

FlexiGene DNA procedure.

Applications

DNA purified using the FlexiGene DNA kit performs well downstream applications, including,

PCR
Southern blotting
Archiving

Supporting data and figures

FlexiGene DNA procedure.

The first step is addition of lysis buffer to the samples. Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC.

Specifications

Features	Specifications
Applications	PCR techniques, restriction digest, Southern blotting, sequencing
Sample amount	100 µl - 20 ml
Format	Scalable
Time per run or per prep	25 - 45 minutes (+ DNA rehydration)
Main sample type	Whole blood, buffy coat, cultured cells
Technology	Single tube precipitation method
Processing	Manual
Yield	35 µg / ml whole blood
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA
Elution volume	200 µl - 1000 µl

Resources

For purification of DNA from human whole blood, buffy coat, cultured cells

This protocol is designed for purification of DNA from up to 10 ml clotted blood using the FlexiGene DNA Kit.

7 years storage at 2-8°C and -20°C

Download Safety Data Sheets for QIAGEN product components.

FAQ

Yes, please follow the User-Developed Protocol 'Purification of DNA from clotted blood using the FlexiGene DNA Kit' (FG01).

FAQ ID -973

Yes. We have shown that DNA purified with the Flexigene DNA Kit is stable for at least 7 years when being stored at either 2-8°C or -20°C. However, the DNA stability is dependent upon storage conditions. The data is available here under Additional Resources.

FAQ ID -1317

Yes, we have the following protocols:

Isolation of genomic DNA from frozen clotted whole blood using the QIAGEN Genomic-tip 100/G (QG02). TEST

You will need to prepare the required buffers according to the recipes in Appendix A of the QIAGEN Genomic DNA Handbook, or you can purchase the Genomic DNA Buffer Set containing pre-made solutions. Alternatively, the QIAGEN Blood & Cell Culture Midi Kit, containing Genomic-tips 100/G and buffers, can be used.

Purification of archive-quality DNA from clotted whole blood using Clotspin Baskets and the Gentra Puregene Blood Kit (PG03).
Purification of archive-quality DNA from clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit (PG04).
Purification of DNA from clotted blood using the FlexiGene DNA Kit (FG01).

FAQ ID -900. Test

Buffer FG3, the hydration buffer supplied in the FlexiGene DNA Kit, is a 10 mM Tris-Cl solution at pH 8.5.

FAQ ID -356