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MagAttract 96 DNA Plant Core Kit (24)
Cat. No. / ID: 67163
MagAttract Suspension and Buffers for 24 x 96 preps
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MagAttract 96 DNA Plant Core Kit
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The MagAttract 96 DNA Plant Core Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- An economical solution
- Reproducible yields of pure, ready-to-use DNA
- A simple, rapid, and reliable procedure
- A manual protocol for initial evaluation
Product Details
The MagAttract 96 DNA Plant Core Kit combines the efficiency of silica-based DNA purification with the convenient handling offered by magnetic particles, in manual, semi-automated, or automated 96-well plate format.
Performance
| Format: | 96-well sample plates |
| Sample sources: | Plant tissue |
| Plant starting material, fresh or frozen: | 10–100 mg |
| Plant starting material, dried or lyophilized: | 10–30 mg |
| Typical yield: | 1–15 µg |
| Elution volume: | 100 µl |
Purified DNA has been used successfully in PCR and real-time PCR (see figures " Successful PCR analysis" and " Reliable real-time PCR analysis").
See figures
Principle
The MagAttract 96 DNA Plant Kit is designed for fully automated, high-throughput preparation of genomic and chloroplast DNA from plant tissue. Proven MagAttract magnetic particle technology in combination with the BioRobot Plant Science system (no longer available) provides efficient and reproducible DNA purification (see figure " Reproducible yields").
See figures
Procedure
Plant samples are mechanically disrupted using a homogenizer such as the TissueLyser. The resulting powder is resuspended in lysis buffer, thoroughly mixed, and centrifuged. Lysates are transferred to a 96-well microplate containing MagAttract magnetic particles, and genomic DNA selectively binds to the surface of the particles (see " MagAttract 96 DNA Plant Core procedure"). After washing, pure genomic DNA is eluted with low-salt buffer, and is ready for use in downstream applications. The MagAttract 96 DNA Plant Kit is designed for use with the BioRobot Plant Science system for fully automated DNA purification. QIAGEN also offers an automated protocol using the BioRobot RapidPlate workstation, and a manual protocol for evaluation purposes.
See figures
Applications
The MagAttract 96 DNA Plant Kit provides high yields of DNA from many plant species. The purified DNA is up to 40 kb in size, with fragments of 23 kb predominating, and is ready to use in downstream applications, including:
- PCR
- Real-time PCR
- Microsatellite analysis
- SNP genotyping
Supporting data and figures
MagAttract 96 DNA Plant Core procedure.
MagAttract 96 DNA Plant Core procedure.
Resources
Kit Handbooks (1)
Safety Data Sheets (3)
Scientific Posters (1)
Certificates of Analysis (1)