Q-Solution Kit

This kit includes Q-Solution and MgCl2 for obtaining better results from PCR reactions with more challenging setups.

S_5730_GEN_QIAGEN_Kits_0810_s

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Q-Solution Kit

Cat. No. / ID:   210220

5x Q-Solution and 25 mM MgCl2 to be combined with any template and assays with any of the QIAcuity master mixes
$88.20
Log in To see your account pricing.
The Q-Solution Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Mitigates the carryover of PCR inhibitors, such as ethanol, heparin and other potential inhibitors 
  • Aids in reactions with nucleic acid templates with complex secondary structures 
  • Improves reactions with carryover of magnesium chelators, such as EDTA 
  • Compatible with QIAGEN kits and instruments for dPCR, end-point PCR and qPCR

Product Details

The Q-Solution Kit has been designed to optimize conditions in challenging qPCR and dPCR reactions. The kit box contains 5x Q-Solution and 25 mM MgCl2, which can be used alone or in combination. If necessary, you can titrate the concentration range of both solutions.

Performance

Minimize effects of PCR inhibitors  

Q-Solution is an innovative and versatile PCR additive, which can be used with any of the QIAcuity master mixes. Carryover of inhibitors such as residual alcohol, heparine, humic acids or other critical agents that may impair the PCR reaction, is mitigated by the special properties of Q-Solution (Figure1).  

 

Boost Primer Binding Affinity 

If you suspect that weak primer or probe binding is resulting in poor PCR performance, try adding MgCl2 to your PCR reaction. 

Compatible with multiple dPCR and qPCR master mixes 

Difficult PCR conditions can exist in any dPCR, end-point PCR or qPCR reaction, delivering  throughput, reproducibility, and sensitivity.. Regardless of your application, data shows that the Q-Solution Kit can enhance dPCR, end-point PCR and qPCR reactions, in some cases after a simple titration of both components.  

Principle

By lowering the melting temperature of the DNA, Q-Solution may overcome difficulties caused by DNA with high-GC content or with complex secondary structures, such as those found in adeno-associated virus (AAV) genomes. The kit also improves PCR efficiency in the presence of inhibitors. MgCl2 addition is optional and prevents PCR failure caused by chelating agents such as EDTA. As an essential cofactor of DNA polymerase, MgCl2 can stabilize double-stranded DNA structures, including those formed between primers and template. The principle of the dPCR reaction in the nanoplates is described here
 

Procedure

The protocol is simple and can be performed in any laboratory with the QIAcuity Digital PCR System. As per the instructions in the user manual, the user can prepare the reaction mix as usual and add Q-Solution and MgCl2 as described in the Quick Start Protocol. The mix is pipetted into the nanoplate. We recommend titrating Q-Solution and MgCl2 before first use to find the optimal concentration for the specific reaction setup.  

Applications

The Q-Solution Kit is highly suitable for various dPCR reactions on the QIAcuity, as well as for end-point PCR and qPCR reactions. For further application examples please check out our Application Note in the “Resources” section.

Supporting data and figures

Resources

Certificates of Analysis (1)
Frequently purchased together
dPCR CGT Assay WPRE  (HEX)
CAT NO./ID 250240
dPCR CGT Assay WPRE (HEX)
For 500x12 µl reactions (20x): QIAGEN Cell and Gene Therapy assay for WPRE to be used with the QIAcuity dPCR system.
$1,317.00
AllTaq Master Mix Kit (2500)
AllTaq Master Mix Kit (2500)
For 2500 x 20 µl PCR amplifications: 10 x 1.25 ml AllTaq Master Mix (4x), 5 x 200 µl Template Tracer (25x), 10 x 50 µl Master Mix Tracer (125x), 20 x 1.9 ml RNase-Free Water
$2,189.00
EZ1 Advanced XL Test Card
CAT NO./ID 9018706
EZ1 Advanced XL Test Card
Preprogrammed card for the EZ1 Advanced XL test protocol
$1,253.00
QIAcuity Probe PCR Kit (5 ml)
QIAcuity Probe PCR Kit (5 ml)
5 x 1 ml 4x concentrated QIAcuity Probe Mastermix, 8 x 1.9 ml Water
$1,223.00
CHO resDNA Quant Standard Kit
CAT NO./ID 250223
CHO resDNA Quant Standard Kit
CHO resDNA Quant Standard (1x), Rehydration Buffer
$1,132.00
Multi Taq2 DNA Polymerase (100)
CAT NO./ID 386315
Multi Taq2 DNA Polymerase (100)
100 Units Multi Taq2 DNA Polymerase
$171.00
QIAamp DNA Accessory Set B
CAT NO./ID 1048146
QIAamp DNA Accessory Set B
Additional buffers and reagents for use with at least 4 x QIAamp DNA Mini Kits (250), cat. no. 51306, on the QIAcube
$177.00
AllTaq PCR Core Kit (5000 U)
AllTaq PCR Core Kit (5000 U)
5 x 200 µl AllTaq Polymerase (5 U/µl), 9 x 1.2 ml AllTaq PCR Buffer (5x), 5 x 200 µl dNTP Mix (10 mM each), 4 x 200 µl Template Tracer (25x), 9 x 50 µl Master Mix Tracer (125x), 5 x 2 ml Q-Solution (5x), 2 x 1.2 ml MgCl2 (25 mM), 16 x 1.9 ml RNase-Free Water
$3,550.00
miRCURY LNA miRNA ISH Buffer and Controls
CAT NO./ID 339459
miRCURY LNA miRNA ISH Buffer and Controls
Scramble miRNA Negative Control Probe, U6 snRNA Positive Control Probe, 2x Formamide-free miRNA ISH buffer, Proteinase K; for 400 slides
$2,057.00
Cell and Gene Therapy Viral Vector Lysis Kit (100 rxn)
CAT NO./ID 250272
Cell and Gene Therapy Viral Vector Lysis Kit (100 rxn)
The kit includes: CGT Sample Stabilizer, DNase I, Nuclease-Free Water, CGT Lysis buffer, CGT DNase I buffer and CGT Dilution Buffer enough to process 100 AAV samples 
$284.00
QIAcuity Nanoplate 8.5k 96-well (10)
CAT NO./ID 250021
QIAcuity Nanoplate 8.5k 96-well (10)
10 QIAcuity Nanoplate 8.5k 96-well, 11 Nanoplate Seals
$1,643.00
Saltonase (5000 U)
CAT NO./ID EN32-050
Saltonase (5000 U)
20 µL x 250 U/µL. Storage temperature: -20°C without a defrost cycle; -80°C for long storage. Stable at -20°C for at least 24 months. No loss in activity is observed up to 6 weeks of incubation at 37°C.
$139.00
PCR Anti-Inhibitor (100)
CAT NO./ID RP50
PCR Anti-Inhibitor (100)
100 μL of PCR Anti-Inhibitor, liquid Storage temperature: -20°C
$56.90
QIAamp Viral RNA Mini QIAcube Kit (240)
CAT NO./ID 52926
QIAamp Viral RNA Mini QIAcube Kit (240)
For 240 viral RNA preps: QIAamp Mini Rotor Adapters, carrier RNA and RNase-free reagents and buffers. Rotor adapters are preloaded with spin columns and elution tubes.
$1,730.00
QIAcuity EG PCR Kit (5 ml)
QIAcuity EG PCR Kit (5 ml)
5 x 1 ml 3x concentrated QIAcuity EvaGreen Mastermix, 5 x 1.9 ml Water
$812.00
QIAcuity Nanoplate 8.5k 24-well (10)
CAT NO./ID 250011
QIAcuity Nanoplate 8.5k 24-well (10)
10 QIAcuity Nanoplate 8.5k 24-well, 11 Nanoplate Seals
$411.00