Custom qBiomarker Somatic Mutation PCR Arrays
For rapid and accurate profiling of somatic mutations using a custom gene panel
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Find or custom design the right target-specific assays and panels to research your biological targets of interest.
Custom qBiomarker Somatic Mutation PCR Arrays
Cat. No. / ID: 337022
PCR plate with a custom panel of mutation assays
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Custom qBiomarker Somatic Mutation PCR Arrays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Configure at GeneGlobe
Find or custom design the right target-specific assays and panels to research your biological targets of interest.
Features
- Profiling of somatic mutations using a custom gene panel
- Simple real-time PCR procedure
- Probe-based assays with high sensitivity and wide dynamic range
- Optimized for robust performance on most PCR instruments
- Master mix included
Product Details
Custom qBiomarker Somatic Mutation PCR Arrays are translational research tools that allow rapid and accurate profiling of somatic mutations using a custom gene panel.
Performance
qBiomarker Somatic Mutation PCR Arrays show a high degree of sensitivity — even with formalin-fixed paraffin-embedded (FFPE) samples (see figures " High degree of sensitivity" and " Sensitivity of qBiomarker Somatic Mutation PCR Arrays with FFPE samples").
qBiomarker Somatic Mutation PCR Arrays are ideal tools for the detection of mutations in cell lines or fresh or FFPE research samples. Sensitive and accurate evaluation of mutation status is critical for toxicological, drug development, and cancer studies (see figure " Profiling of common cancer cell lines for somatic mutation status").
See figures
Principle
Real-time PCR is the most sensitive and reliable method for the detection of DNA mutations. By combining allele-specific amplification and hydrolysis probe detection, qBiomarker Somatic Mutation real-time PCR assays have been developed which can detect as few as 1% somatic mutations in the background of wild-type genomic DNA. Allele-specific amplification is achieved by Amplification Refractory Mutation System (ARMS) technology, which is based on the discrimination by Taq polymerase between a match and a mismatch at the 3’ end of the PCR primer (see figure " Principle of ARMS technology").
See figures
Procedure
Simply mix the DNA template with the ready-to-use PCR master mix, aliquot equal volumes to each well of the same plate, and then run the real-time PCR cycling program. qBiomarker Somatic Mutation PCR Arrays are compatible with all ABI, Bio-Rad, Eppendorf, Roche, and Stratagene instruments.
qBiomarker Somatic Mutation PCR Arrays are available in 96-well and 384-well plates and are used to detect mutations related to a disease state or pathway, plus gene copy number controls for normalization. Each qBiomarker Somatic Mutation PCR Array also includes control elements for general PCR performance.
Easy-to-use tool for data analysis
Data can be analyzed using the available Excel-based data analysis templates. Data analysis is based on either the ∆∆CT or Average ∆CT method.
Arrays are available in a variety of formats, all with mastermix included:
Applications
Custom qBiomarker Somatic Mutation PCR Arrays are highly suited for the rapid and accurate profiling of mutations for a custom panel of genes.
Supporting data and figures
Profiling of common cancer cell lines for somatic mutation status.
200 ng gDNA from wild-type or cancer cell lines had 85 mutations (x axis) profiled on the Human EGFR qBiomarker Somatic Mutation PCR Array. Each detected mutation is indicated as a peak. All previously identified mutations in the EGFR pathway in these cell lines were confirmed.
Resources
Supplementary Protocols (7)
Safety Data Sheets (1)
Certificates of Analysis (1)