QIAcuity Nanoplates und Zubehör

Name: QIAcuity Nanoplates und Zubehör
Brand: Qiagen

Zur Verwendung mit den QIAcuity digitalen PCR-Geräten

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QIAcuity Nanoplate 26k 8-well (10)

Kat.-Nr. / ID. 250031

10 QIAcuity Nanoplate 26k 8-well, 11 Nanoplate Seals

306,00 $

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Product TypeAccessories

QIAcuity Nanoplate

Nanoplate Seals

Nanoplate Tray

Nanoplate Adapter

Partitionen pro Well

26k

8.5k

Anzahl Wells

Menge

QIAcuity Nanoplates und Zubehör sind für molekularbiologische Anwendungen vorgesehen. Diese Produkte sind nicht zur Diagnose, Prävention oder Behandlung einer Erkrankung vorgesehen.

✓ Automatische Verarbeitung von Online-Bestellungen 24/7

✓ Sachkundiger und professioneller technischer und Produkt-Support

✓ Schnelle und zuverlässige (Nach-)Bestellung

Eigenschaften

Vier Nanoplatten für unterschiedliche Anwendungsbereiche
Bis zu 8.500 oder 26.000 Partitionen je Kavität
SBS-Format

Angaben zum Produkt

Die QIAcuity Nanoplates sind Platten für die mikrofluidische digitale PCR, die die Analyse von 8, 24 oder 96 Proben mit bis zu 8.500 oder 26.000 Partitionen je Kavität ermöglichen. Alle vier Nanoplatten sind für den Einsatz auf QIAcuity Digital-PCR-Geräten optimiert.

Die Nanoplatten können ausschließlich mit dem QIAcuity Digital PCR System verwendet werden. Für das automatisierte Flüssigkeitsmanagement und PCR-Setup mit einer QIAcuity Nanoplate auf dem QIAgility wird der spezielle QIAcuity Nanoplate Adapter benötigt. Laden Sie die fertig befüllte Platte in das QIAcuity Digital PCR System.

Möchten Sie Näheres über das Produkt erfahren und von einem unserer dPCR-Spezialisten kontaktiert werden? Registrieren Sie sich hier, und wir werden uns umgehend mit Ihnen in Verbindung setzen.

Leistung

Dies sind speziell für die digitalen PCR-Reaktionen in den QIAcuity Geräten entwickelte Platten. QIAGEN bietet vier Nanoplattentypen an, alle im SBS-Format, aber mit unterschiedlichen Spezifikationen für verschiedene Anwendungsbereiche.

Nanoplate – Eigenschaften und technische Spezifikationen
Typ	Rahmenfarbe	Spezifikationen	Anwendungen
Nanoplate 26K 8-Well	Hellblau	8-Well x rund 26.000 Partitionen 40 µl dPCR-Reaktion je Kavität	Nachweis seltener Mutationen, Flüssigbiopsie, Genexpressionsanalyse, Nachweis von Krankheitserregern usw.
Nanoplate 26K 24-Well	Blau	24-Well x rund 26.000 Partitionen 40 µl dPCR-Reaktion je Kavität	Nachweis seltener Mutationen, Flüssigbiopsie, Genexpressionsanalyse, Nachweis von Krankheitserregern, usw.
Nanoplate 8.5K 24-Well	Weiß	24-Well x rund 8.500 Partitionen 12 µl dPCR-Reaktion je Kavität	Analyse von Kopienzahlvariationen, Genexpressionsanalyse, Quantifizierung von NGS-Bibliotheken, Erkennung von Genomveränderungen, usw.
Nanoplate 8.5K 96-Well	Grau	96-Well x rund 8.500 Partitionen 12 µl dPCR-Reaktion je Kavität

Prinzip

In 3 einfachen Schritten erhalten Sie in weniger als 2 Stunden das gewünschte dPCR-Ergebnis: pipettieren und laden, Experiment durchführen, Ergebnisse auswerten. Das Prinzip der dPCR-Reaktion in den Nanoplatten finden Sie hier beschrieben.

Verfahren

Genau wie bei qPCR-Experimenten umfasst die Probenvorbereitung die Überführung von Master-Mix, Sonden und Primern in eine 8-, 24- oder 96-Well-Nanoplatte, gefolgt von der Zugabe der Proben. Das System integriert Partitionierung, Thermocycling und Bildgebung in nur einem vollautomatischen Gerät, mit dem Sie in weniger als 2 Stunden von der Probe zum Ergebnis gelangen. Mit der Software Suite lassen sich Auswertungen durchführen, die die Konzentration der Zielsequenz in Kopien pro Mikroliter sowie Qualitätskontrollen wie positive Proben und NTC liefern. Diese Auswertung kann auch auf Remote-Computer innerhalb desselben lokalen Netzwerks (LAN) ausgedehnt werden.

Anwendungen

Im Zusammenspiel mit dem QIAcuity Digital PCR System und den QIAcuity PCR Kits ermöglichen die QIAcuity Nanoplates digitale PCR-Anwendungen wie z. B.:

Nachweis seltener Mutationen
Auswertung der Variation der Kopienzahl
Genexpressionsanalyse
Pathogennachweis
Genotypisierung
miRNA-Forschung
Zell- und Gentherapie
Quantifizierung von Rest-DNA
Abwasserüberwachung

Ressourcen

For Version 2.0

Version 1.1.3

For Version 2

Version 4.0

The Volume Precision Factor (VPF) offers a unique feature to secure precision of concentration results obtained from a QIAcuity dPCR run.
In general, Nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. Potential variation of partition sizes in Nanoplate batches, caused by different microstructure molding forms, can be addressed by applying the batch specific VPF. Furthermore, the VPF includes well-specific volume information and therefore further increases precision of concentration calculation in each well of the Nanoplates.

After downloading and updating the VPF file within the QIAcuity Software Suite, the VPF is applied automatically to the analysis of a corresponding Nanoplate batch. The VPF file includes information from all available microstructure molding forms and connected Nanoplate batches. It will be stored on the PC where the QIAcuity Software Suite is installed.

Required QIAcuity Software Suite version: Version 1.2 or higher.

Version 2.1

Beside of usability improvements and bug fixes, the new features address GMP requirements by offering additional support for 21 CFR part 11 compliance, copy number variation (CNV) analysis, support of future Nanoplates, and improved 1D scatterplot views.

For more information, please refer to the Release Note: QIAcuity Software Suite, Version 2.1

SHA1 checksum: 6D51E273B26FA7D7E0EDA843CB3DCD74B38FA5B1

Version 2.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis

Besides system stability and performance reliability improvements, this new version of the control software offers advanced user management and audit trail functionalities. Together with the QIAcuity Software Suite 2.0, the QIAcuity systems now support 21 CFR Part 11 regulations for users working under GMP.

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Note: Upgrading the QIAcuity Control Software to version 2.0 will require upgrading the QIAcuity Software Suite to version 2.0.

Important: Please follow the instructions provided in the QIAcuity User Manual for software version 2.0. It is strongly recommended to update the QIAcuity Software Suite first before proceeding with the QIAcuity Control Software!

Version 1.0

The QIAcuity Control Software is an integral part of the QIAcuity instrument. It offers a GUI (graphical user interface) for basic functionalities such as plate setup, changing the order of plates to be processed, and monitoring the status of runs in real time. After a run is completed, the data are stored on the instrument’s memory and are sent to the connected QIAcuity Software Suite for analysis.

This new version of the control software includes system stability and performance reliability improvements. We highly recommend to update the control software of your QIAcuity instrument.

Note: The installation requires approximately 90 minutes.

Version 2.0

The QIAcuity Software Suite 2.0 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

This new software version now supports 21 CFR Part 11 regulations for users working under GMP. This includes the following:

-Advanced User Management
-Audit Trail
-Signatures

Additional changes compared with the previous software version include the following:

-Usability improvements
-Improved dust detection
-Hyperwell expansion to CNV and GEX analysis
-CSV export functionality of multiple positive partitions for linkage analysis, drop-off assays, etc.
-Raw data export

Detailed information is available in the Release Note, which can also be downloaded under section “Operating Software”.

Important: A direct update of the Software Suite from version 1.1.3 to 2.0.20 is not possible. Please follow the instructions in the User Manual on how to update to version 1.2.18 first before updating the Software Suite to version 2.0.20.

Before you start upgrading to version 1.1.3, please make sure that all plates are imported and visible in the plate overview of the QIAcuity Software Suite.

Caution: Not following the instructions may result in a loss of your previous plate data!

Please contact QIAGEN Technical Services if you are unsure and require technical support.

SHA1 checksum: C60C4EEC349DF1C037796016D32A93EEC19B54A0

Version 2.1

Beside of improvements and bug fixes, the new features mainly address the GMP requirements by offering additional support for 21 CFR part 11 compliance and get the software ready for future Nanoplate support.

For more information, please refer to the Release Note: QIAcuity Instrument Control Software, Version 2.1.

SHA1 checksum: 0DD41A8DE46D10E81F538ACB71AC184170D39FE0

Version 1.2

The QIAcuity Software Suite 1.2 is designed to be installed on a Windows PC that is connected to one or more QIAcuity instruments. The QIAcuity Software Suite enables the user to set up plates, analyze results, and monitor the status of runs in real time. For this configuration, the QIAcuity instrument needs to be connected to a network through Ethernet. Alternatively, a direct cable connection between the QIAcuity and the notebook where the QIAcuity Software Suite is running needs to be established. When connected to a network, up to 10 users may access the QIAcuity Software Suite via a browser installed on the client PC (Windows or Mac).

The following browsers are supported in the QIAcuity Software Suite:

-Mozilla Firefox (version 64.0.2 or higher)
-Microsoft Edge (version 44.17763.1.0 or higher)
-Google Chrome (version 71.0.3578.98 or higher)

The new QIAcuity Software Suite 1.2 offers a functionality that enables users of the QIAcuity Software 1.1.3 to upgrade to the new version while keeping the library of previously stored plate runs.

Note: If you have exported plates from QIAcuity Software Suite 1.1.3 that you would like to import and use in QIAcuity Software Suite 1.2, you will need to import the plates before upgrading from version 1.1.3 to version 1.2. You may then export the plates again. Future software version starting from QIAcuity Software Suite 2.0 will facilitate import of plates from previous QIAcuity Software Suite versions.

The new improvements are as follows:

-Support for the Nanoplate 8.5k 24-well
-Hyperwell functionality to combine several wells to one combined well for analysis
-Automated plate archiving functionality
-Functionality to show the number of single/double positives in 2D scatterplots
-VPF (Volume Precision Factor) to further improve concentration calculation (see related resources)
-Additional improvements for stabilization and troubleshooting

For Version 1.2

For Version 1.0

The QIAgility instrument is a liquid handler designed for automating PCR setup. For compatibility with the QIAcuity, we developed an adapter to secure up to two nanoplates onto the deck of the QIAgility. Using the QIAgility software, we have optimized a protocol that works for all nanoplate types and QIAcuity applications. Here we report the performance of a front end automated QIAgility dPCR nanoplate setup procedure for use with the QIAcuity dPCR system.

The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.

Digital PCR is a superior method to qPCR for the detection and absolute quantification of low concentration target templates. There are multiple digital PCR systems on the market that differ in numerous aspects including the amount of dead volume, which is the volume that is loaded but not analyzed by the given instrument. While it has been speculated that dead volume could impact the sensitivity of dPCR applications, here we provide data to support the conclusion that the most important factors in determining the relative sensitivity of each system are template addition volume and template analyzed volume. In summary, data provided herein demonstrate that higher template addition volumes can overcome any limitations that dead volume may have on the sensitivity of a dPCR application.

The goal of this work was to compare performance of quantitative PCR (qPCR) and digital PCR (dPCR) in the quantification of gene expression and Wolbachia abundances in Nasonia parasitoid wasps.

This study tested a workflow for quantitation and qualification of AAV samples using a duplex assay on the QIAcuity dPCR instrument targeting both an insert (GFP) and the viral backbone (AAV2-ITR). With very low intra-assay and inter-assay CVs <6.5%, we demonstrate one of the main benefits of dPCR: reproducibility.

Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.

Here we demonstrate how to optimize your assays on a microfluidic nanoplate-based digital PCR system, the QIAcuity, and provide recommendations for a seamless transfer. Moreover, the QIAcuity dPCR workflow is very similar to qPCR.

Here we compared the performance of qPCR and the nanoplate dPCR techniques. The digital PCR method on the QIAcuity significantly improved precision when measuring copy number states and sensitivity of mutation detection through absolute quantification and reduced standard error. This is advantageous in various applications, including copy number variation analysis, small fold-change and rare mutation detection.

Here we provide an integrated rAAV genome titer method using the QIAcuity Digital PCR (dPCR) System with detailed parameters for high assay performance. Using this optimized method for pre-PCR handling of in-process rAAV samples, the results demonstrated that QIAcuity dPCR system generates the same level of accuracy and precision as the current gold standard ddPCR system but with much faster sample-to-result times (2 hours vs 7 hours) and higher overall throughput and scalability.

Fast. Scalable. Reliable.

For use with QIAcuity systems

July 2021

FAQ

This is not needed. The QIAcuity is equipped with a flexible power supply technology and operates within a range of 100–240V AC, 50/60 Hz, 1500 VA (max).

FAQ-3761

The instrument software GUI shows error codes including a description and information how to resolve the error. The instrument touchscreen shows an alarm icon in the upper right corner that turns red in case of an instrument failure. Accessing the System Status in the Tool tab allows users to clear errors. Rebooting of the instrument is required to complete the removal of the error. Please do not skip this step. You may always contact QIAGEN Technical Services in case of any question.

FAQ-3763

The user manual contains instructions on how to perform a regular cleaning and decontamination, and how to replace air filters on the QIAcuity instruments. A regular maintenance reduces the dust in the instrument and therefore minimizes the presence of dust particles on the nanoplate, which might interfere with the plate analysis.

FAQ-3765

If you had run a nanoplate for which the installed VPF misses the specific factor, the software will notify you. If you then analyze without the specific VPF, the impact depends on the variation of the partition volume of the new Nanoplate batch compared to the latest. Typically this variation is ±6–7% (approx. 5% CV over the entire plate). The analysis may be repeated after updating the VPF file. After installing the latest VPF and re-analysis of the run, a copy of the plate is generated in the QIAcuity Software Suite including the new results.

FAQ-3769

Yes, the report includes a notification if the matching VPF was missing and, therefore, not applied to the analysis. If the matching VPF was applied there is no notification on the report.

FAQ-3770

If you had analyzed your nanoplates without VFP, the impact depends on the variation of the partition volume of the new nanoplate batch compared to the latest. The VPF reduces the CV from approximately 5% to 2%. We recommend to reanalyze results in case the data originated from different wells (e.g., copy number variations or gene expression data sets for which the reference sample was measured in a different well). Results obtained across different plates should also be r-analyzed. A reanalysis is not required for assay data that were analyzed within the same well (e.g., mutation rate determination using two channels within the same well).

FAQ-3771

A standard PCR plate is required to set up dPCR reaction before transferring it to the nanoplate to ensure a proper mixing of the reaction mix before partitioning.

FAQ-3774

QIAGEN master mixes are optimized for nanoplate microfluidics and are recommended to be used with our dPCR system. They also include an optimized reference dye required for proper analysis.

FAQ-3777

All DNA samples used in reaction mixes should show similar quality and quantity, which can easily be assessed using UV spectrophotometry. DNA samples with an average length of ≥20 kb (e.g., genomic DNA purified via spin column with silica membrane) should be fragmented by restriction digestion before partitioning. Enzymatic fragmentation of larger DNA ensures an even distribution of template throughout the QIAcuity Nanoplate, which in turn leads to an accurate and precise quantification.

FAQ-3778

The QIAcuity Probe PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity Probe PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 12 months, unless otherwise indicated on the label.
The QIAcuity EG PCR Kit should be stored immediately upon receipt at –30 to –15°C in a constant-temperature freezer and protected from light. The QIAcuity EG PCR master mix can also be stored protected from light at 2–8°C. Components are stable for 6 months, unless otherwise indicated on the label.
The QIAcuity Nanoplates does not have expiry date and are stable for at least 1 year when stored at RT.

FAQ-3780

The plate is designed for a single use run. For example, even if only 30 samples are loaded into the 96-well plate, a whole plate will be sealed by the roller. It can't be unsealed and used for another run. The QIAcuity Software won’t allow to set up a separate experiment for the same nanoplate to avoid that previously processed plates being not partitioned a second time.

FAQ-3781

An essential temperature gradient functionality was introduced with software version 2.5. When updating older software versions to 2.5, each QIAcuity instrument will offer the temperature gradient and may be used to find the best cycling temperature for new dPCR assays. When running a QIAcuity Four or a QIAcuity Eight, all plates may have their own temperature profile, including the option for a temperature gradient.

FAQ-3783

The VPF provides a set of well-specific and molding form-specific factors used to specify the exact reaction volume of a nanoplate, thus increasing the concentration calculation of each well.

FAQ-3784

In general, nanoplates provide partitions of fixed sizes that enable a very precise way of sample concentration calculation. If a new molding form is used for nanoplate manufacturing, potential variation of partition sizes can be addressed by applying the molding form-specific VPF. Thus, each time a new molding form is used, a new VPF is created and made available. Currently, the VPF is updated once every 3–6 months.

FAQ-3785

In dPCR we measure the absolute concentration of targets at endpoint reaction. Concentrations of unknowns can be determined based on dPCR results observed (number of negatives, number of positives, and partition volume analyzed).

FAQ-3786

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