Why is a 2-step (and not a 3-step) cycling protocol recommended for QuantiFast SYBR Green PCR Kits?

The QuantiFast SYBR Green RT-PCR Kit delivers fast and specific quantification of RNA targets by real-time one-step RT-PCR using SYBR Green I detection. Q-bond technology and an optimized, ready to use master mix enable shorter real-time RT-PCR run times, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique qRT-PCR buffer system ensures highly specific and sensitive real-time quantification of RNA targets. The QuantiFast SYBR Green RT-PCR Kit is also supplied with an optimized RT mix for efficient cDNA synthesis in only 10 minutes. For convenience, the master mix can be stored at 2–8°C. IMPORTANT NOTE: As announced earlier, the production of the QuantiFast kits has been discontinued since mid-2021. Hence, these products will be available only until stocks last. Visit the product page of the successor kit to view improved features or to request a trial kit. For more information and FAQs on this transition, visit: www.qiagen.com/PCRresource.

The QuantiFast SYBR Green PCR Kit delivers fast and specific quantification of gDNA or cDNA targets by real-time PCR or two-step RT-PCR using SYBR Green I detection. Q-bond technology and an optimized, ready-to-use master mix enable shorter real-time PCR run times, not only on fast cyclers with short ramping times, but also on standard cyclers. The combination of a hot start and a unique PCR buffer system in the ready-to-use master mix ensures highly sensitive qPCR on any real-time cycler without the need for optimization. For convenience, the master mix can be stored at 2–8°C. IMPORTANT NOTE: As announced earlier, the production of the QuantiFast kits has been discontinued since mid-2021. Hence, these products will be available only until stocks last. Visit the product page of the successor kit to view improved features or to request a trial kit. For more information and FAQs on this transition, visit: www.qiagen.com/PCRresource.