FAQ-2382
Is V600E the only mutation that can be detected with the codon 600 assay in the BRAF Pyro Kit?
No, the V600E is not the only mutation that can be detected with the BRAF Pyro Kit. The sequence to analyze can be changed post-run to allow detection also of V600M (GTG>ATG), V600A (GTG>GCG) and V600G (GTG>GGG).
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BRAF Pyro Kit
The BRAF Pyro Kit is a molecular detection kit for the identification of mutations in the BRAF gene. The kit provides primers and reagents for amplification of the BRAF gene, plus buffers, primers, and reagents for detection and quantification of mutations in real time using Pyrosequencing technology on the PyroMark Q24 System.