FAQ-2724
Can miScript miRNA qPCR Assays be used for pre-miRNA detection?
No. miScript miRNA PCR Arrays and miScript Primer Assays are designed to measure exclusively the expression of mature miRNA, not pre-miRNA. To measure miRNA precursors use miScript Precursor Assays available through the QIAGEN GeneGlobe assay search portal.
Related products
miScript miRNA PCR Arrays
miScript miRNA PCR Arrays are mature miRNA-specific forward primers (miScript Primer Assays) that have been arrayed in biologically relevant pathway-focused, disease-focused, or whole miRNome panels. miScript miRNA PCR Arrays are part of the miScript PCR System for miRNA detection and quantification. cDNA is prepared using the miScript II RT Kit with miScript HiSpec Buffer and is used as a template in real-time PCR with a miScript miRNA PCR Array and the miScript SYBR Green PCR Kit. Important note: This product line will be discontinued on May 1, 2021 and will be replaced by the miRCURY LNA miRNA PCR system. We strongly recommend using the following miRCURY LNA PCR products for better miRNA quantification and profiling: miRNome PCR Panels, miRNA Focus PCR Panels and miRNA QC PCR Panel. For more information about this transition, please visit our dedicated knowledge hub or contact us.
Pathway-focused miScript miRNA PCR Arrays
miScript miRNA PCR Arrays are mature miRNA-specific forward primers (miScript Primer Assays) that have been arrayed in biologically relevant pathway-focused, disease-focused or whole miRNome panels. miScript miRNA PCR Arrays are part of the miScript PCR System for miRNA detection and quantification. cDNA is prepared using the miScript II RT Kit with miScript HiSpec Buffer and is used as a template in real-time PCR with a miScript miRNA PCR Array and the miScript SYBR Green PCR Kit. Important note: This product line will be discontinued on May 1, 2021 and will be replaced by the miRCURY LNA miRNA PCR system. We strongly recommend using the following miRCURY LNA PCR products for better miRNA quantification and profiling: miRNome PCR Panels, miRNA Focus PCR Panels and miRNA QC PCR Panel. For more information about this transition, please visit our dedicated knowledge hub or contact us.