Will pipetting error affect the EpiTect Methyl qPCR Array results?

The passive reference dyes in the PCR SYBR Green master mixes, such as ROX and Fluorescein, are used by the real-time PCR instruments to normalize variation from well to well. Therefore, these systems tolerate volume variations caused by pipetting error or evaporation. Even a 20% pipetting error causes only 0.05-cycle differences in Ct values.