FAQ-2766
The pathway reporter luciferase activity values are greater than the positive control, is there a problem?
No, the positive control only serves as a constitutively-active reporter enabling the user to assess if the transfection/transduction was successful, and should not be used for direct comparison to the pathway reporters.
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Cignal Reporter Controls
Cignal Reporter Assays provide a rapid, sensitive, and quantitative assessment of signal transduction pathway activation by measuring the activities of downstream transcription factors. When conducting experiments using Cignal Reporter Assays, proper controls are a key element of the experimental design. They permit accurate interpretation of reporter assay results and provide assurance of the specificity of the observed response.