FAQ-2989
What sample types should be processed with the low content (LC) and high content (HC) protocols for DNA extraction on the QIAsymphony SP?
Tissues will give increased DNA yields when processed with the high content protocol, but the low content protocol in combination with a small elution volume (50µl) may be used if high DNA concentration is required. For FFPE tissue we recommend using the low content protocol.
Related products
QIAsymphony DSP DNA Kits
QIAsymphony DSP DNA Kits, in combination with the QIAsymphony SP, enable automated total DNA purification from human whole blood, buffy coat, tissues and FFPE tissues, as well as viral DNA from human whole blood for in vitro diagnostic use. Kits are available in mini and midi formats for sample volumes ranging from 200 µl to 1000 µl. For tissue and FFPE tissue protocols, manual sample pretreatment is required. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.
QIAsymphony SP/AS instruments
The QIAsymphony SP is a versatile nucleic acid purification instrument providing high-quality nucleic acids compatible with with most common downstream assay technologies, such as real-time PCR, digital PCR, and next generation sequencing (NGS). The large range of a dedicated QIAsymphony kits enables optimized purification of genomic DNA, cell-free circulating DNA, RNA, bacterial and viral nucleic acids from a wide range of starting materials. The QIAsymphony AS extends the capabilities of the QIAsymphony SP by integrating automated PCR assay setup, which, in combination with the Rotor-Gene Q and QIAGEN real-time and end-point PCR kits, enables you to optimize your PCR workflow and maximize your efficiency.