FAQ-3463
How long can the tubes be kept frozen at –20°C or –70°C?
Currently we have data for storage at –20°C and –70°C for 11 years.
Long term archiving studies are ongoing. See technical note: In Situ Stability of RNA in Blood Samples Stored at –20°C and –70°C in PAXgene Blood RNA Tubes.
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PAXgene Blood RNA Kit IVD
The PAXgene Blood RNA system consists of PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for blood collection, stabilization, and transport, and the PAXgene Blood RNA Kit for silica-membrane-based RNA isolation and purification in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube Connect MDx. The system has received FDA marketing clearance and provides exact performance specifications for in vitro diagnostic use.
PAXgene Blood RNA Tubes (IVD)
The PAXgene Blood RNA System consists of a blood collection tube (PAXgene Blood RNA Tube) and a nucleic acid purification kit (PAXgene Blood RNA Kit). It is intended for the collection, storage and transport of blood and the stabilization of intracellular RNA in a closed tube, and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.
Performance characteristics for the PAXgene Blood RNA System have only been established with FOS* and IL1B** gene transcripts. The user is responsible for establishing appropriate PAXgene Blood RNA System performance characteristics for other target transcripts.
*FOS is the gene symbol for human Fos Proto-Oncogene, AP-1 Transcription Factor Subunit.
**IL1B is the gene symbol for human Interleukin 1 Beta.