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Get in touch with our team today and request a quote to trial the EZ2 RNA FFPE Kit (48).
EZ2 RNA FFPE Kit (48)
Cat no. / ID. 959734
For 48 preps: EZ2 RNA FFPE cartridge, disposable filter-tips and tip-holders, tubes, Paraffin Removal Solution, DNase Booster, RNase-free DNase I, RNase-free water
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The EZ2 RNA FFPE Kit (48) is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The EZ2 RNA FFPE Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Want to try this solution for the first time?
Get in touch with our team today and request a quote to trial the EZ2 RNA FFPE Kit (48).
Features
- Recovers large amounts of amplifiable RNA
- Removes paraffin without using xylene or similar solvents
- Pretreatment-to-elution workflow is fully automated on the EZ2 Connect
- End product is highly suitable for downstream real-time RT-PCR, NGS or digital PCR (dPCR)
Product Details
The EZ2 RNA FFPE Kit purifies large amounts of amplifiable RNA from formalin-fixed, paraffin-embedded (FFPE) tissue samples. This kit's protocol is fully automated from pretreatment to elution using the EZ2 Connect.
Principle
There are two ways that the EZ2 RNA FFPE Kit makes RNA extraction more efficient: First, it removes paraffin without multiple wash steps. This reduces the risk of losing sample material and the RNA they hold. Second, it fully automates all procedures after the deparaffinization step, so the number of tedious hands-on steps are radically reduced.
Procedure
To remove paraffin from the FFPE tissue sample, add Paraffin Removal Solution to the sample sections, vortex for 10 seconds, centrifuge briefly, then incubate for 3 minutes. Your manual procedure ends here.
Transfer the tubes containing the sample sections into the EZ2 Connect, set up and start the run (see “ Fully automated, from pretreatment to elution”).
If you enabled QIAsphere connectivity in your EZ2 Connect, you will receive a notification on your desktop or mobile device when the run is finished.
See figures
Applications
The EZ2 RNA FFPE workflow is largely automated. All steps except deparaffinization actually happen during the EZ2 Connect run. When the run is over, the eluate that you take out of the instrument is suitable for downstream real-time RT-PCR, dPCR or NGS.
Supporting data and figures
Fully automated, from pretreatment to elution.
Add Paraffin Removal Solution to the sample sections. Vortex, centrifuge and incubate. Then transfer the samples to the EZ2 Connect to complete the workflow.
Specifications
| Features | Specifications |
|---|---|
| Applications | RT-PCR, dPCR, NGS |
| Main sample type | FFPE tissue samples |
| Elution volume | 50 or 100 µl |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | RNA |
| Processing | Automated with an EZ2 Connect instrument |
| Number of preps per run | 1–24 samples per run |
| Technology | Magnetic particle technology |
| Sample amount | Tissue sections, each with a thickness of 5–10 µm, for a total volume of 4 mm3 |
Resources
Quick-Start Protocols (1)
Kit Handbooks (1)
Safety Data Sheets (1)
Certificates of Analysis (1)
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