QIAcuity One-Step Viral RT-PCR Kit

供QIAcuity 数字 PCR 仪器使用

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QIAcuity One-Step Viral RT-PCR Kit

Cat. No. / ID:   1123145

4x 1.3 ml One-Step Viral RT-PCR 预混液 (4x),2x 100 μμLMultiplex Reverse Transcription Mix (100x) 和 8x 1.9 ml RNase-Free 水; 26K 纳米微孔板中可进行 500 个反应,8.5K纳米微孔板中可进行 1,666 个反应
QIAcuity One-Step Viral RT-PCR Kit 将于 2023 年 2 月后逐步淘汰。我们建议尽早更换为改进后的产品 QIAcuity OneStep Advanced Probe Kit
QIAcuity One-Step Viral RT-PCR Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

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Features

  • 适用于单重和最多 5 重反应
  • 针对在 QIAcuity 纳米微孔板中的微流体使用优化
  • 4x 浓缩 PCR 预混液,可加载更多样本

Product Details

QIAcuity One-Step Viral RT-PCR Kit 可让您在 QIAcuity 数字 PCR 仪器上对 RNA 靶标进行高度灵敏的定量。

该试剂盒包含4倍浓缩PCR预混液,用于QIAcuity纳米微孔板。该试剂盒提高了探针法数字 PCR 的特异性和效率,可提供准确的单一或最多 5 重的定量分析。

Performance

优越的性能
适用于水解探针基 dPCR 的 QIAcuity One-Step RT-PCR 预混液采用了 QIAGEN 的最新版高品质 DNA 聚合酶。QIAGEN 的专有技术与经过优化验证的专门针对纳米微孔板微流体优化的缓冲液技术的独特结合可保障在灵敏度、可重复性及效率等方面高度一致的结果。

使用 QIAcuity Probe PCR Kit 进行单重到五重检测
QIAcuity Probe PCR Kit 中的独特预混液可让您在一个 QIAcuity 纳米微孔板孔中对最多 5 个丰度不同的靶标进行准确定量。这可节省时间和金钱,降低所需的样本材料用量。此外,获得的双重或多重 PCR 数据可与从单重 PCR 中获得的数据相比。

Principle

QIAcuity One-Step Viral RT-PCR Kit 包含一份即用型 4x PCR 预混液,无需优化反应及循环条件。
纳米微孔板中 dPCR 反应的原理说明,请参见这里。

Procedure

QIAcuity One-Step Viral RT-PCR Kit 包含一份即用型 4x PCR 预混液,无需优化反应及循环条件。仅需将 100x RT 混合物、模板 RNA 和引物探针组添加到预混液中并执行快速启动方案,就能在 QIAcuity 上快速获得可靠的结果。

Applications

QIAcuity One-Step Viral RT-PCR Kit 与 QIAcuity Digital PCR System 和 QIAcuity 纳米微孔板的结合使用可让您在各种应用(包括但不限于病毒 RNA 检测)中进行 RNA 靶标的定量分析。

Resources

应用说明 (2)
The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.
Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.
快速启动实验方案 (1)
用户使用手册 (1)
For use with QIAcuity systems

July 2021
Application Notes (2)
Here we report the use of saliva samples in combination with dPCR as a suitable alternative to screen for individuals infected with SARS-CoV-2.
The QIAcuity digital PCR system combined with the QIAcuity One-Step Viral RT-PCR Kit enables precise detection and quantitation of vector-borne viruses in mosquitoes. The results presented in this comparison study showed that digital PCR is a powerful tool for absolute quantitation of low abundant targets and is a more reliable detection method than qPCR. Multiplexing allows detection and quantitation of multiple targets in a single reaction more efficiently by increasing sample throughput at a reduced cost per target.
Certificates of Analysis (1)
Instrument User Manuals (1)
For use with QIAcuity systems

July 2021
Quick-Start Protocols (1)