ipsogen BCR-ABL1 mbcr Kit

采用real-time PCR对BCR-ABL mbcr e1a2转录本进行定量检测

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ipsogen BCR-ABL1 mbcr Kit (24)

Cat. No. / ID:   670013

For 24 reactions: ABL Control Gene Standards, BCR-ABL mbcr Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix BCR-ABL mbcr Fusion Gene
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仅限于科研应用,不适用于诊断,不为疾病的诊断、预防或治疗提供参考信息。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 灵敏、准确的定量检测试剂
  • 符合EAC要求的标准化试剂,结果具有可重复性
  • 即用型解决方案,便利的工作流程

产品详情

ipsogen BCR-ABL1 mbcr Kit仅适用于科研,不可用于诊断。该试剂盒可用于Rotor-Gene Q实时荧光定量PCR分析仪或其他real-time PCR仪的real-time PCR实验。该试剂盒中的试剂经过优化,能够对BCR-ABL mbcr p190 e1a2转录本在总RNA中相对于ABL对照基因的表达进行可靠、灵敏的定量检测。

绩效

为确保高灵敏度,ipsogen BCR-ABL1 mbcr Kit已经过优化,使用预稀释的质粒标准品和引物探针混合液,检测BCR-ABL mbcr p190 e1a2和ABL转录本(参见 Accurate detection of BCR-ABL mbcr plasmid standards Reliable detection of ABL plasmid standards)。
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原理

ipsogen BCR-ABL1 mbcr Kit为即用型试剂盒,采用real-time PCR技术,检测BCR-ABL mbcr p190 e1a2转录本。该试剂盒可扩增并检测总RNA中相对于ABL对照基因表达的特定BCR-ABL mbcr p190 e1a2转录本(参见 BCR-ABL mbcr fusion gene transcript ABL control gene transcript)。该试剂盒灵敏度高,特异性强,可重复性好。ipsogen BCR-ABL1 mbcr Kit为BCR-ABL mbcr提供5个标准品稀释液,为ABL提供3个标准品稀释液。使用标准品能够实现准确的定量检测。BCR-ABL mbcr定量技术已根据欧洲抗癌协会(EAC)的推荐进行了标准化,ipsogen BCR-ABL1 mbcr Kit采用经验证的技术来校准结果。
查看图表

程序

第一步是将样本中的总RNA逆转录为cDNA。第二步是通过real-time PCR扩增cDNA。ipsogen BCR-ABL1 mbcr Kit可对BCR-ABL mbcr p190 e1a2和ABL基因转录本进行定量检测。只需按照试剂盒操作手册中的实验方案,即可便利的开始反应。

应用

ipsogen BCR-ABL1 mbcr Kit能够对BCR-ABL mbcr p190 e1a2转录本相对于ABL对照基因的表达进行可靠、灵敏的定量检测。仅适用于科研。

辅助数据和图表

资源

操作软件 (1)
To download Rotor-Gene AssayManager v2.1.1 after purchase:
    Click the Rotor-Gene AssayManager v2.1.1 Software header. On the next window, click Continue. Follow the on-screen instructions to install Rotor-Gene AssayManager v2.1.1.
    Rotor-Gene AssayManager v2.1.1 and license must be purchased prior to use.    

    IMPORTANT: In order to perform and analyze runs, at least one Rotor-Gene AssayManager v2.1.1 plug-in needs to be installed. Refer to your assay kit handbook for more information.

    IMPORTANT: To allow checksum integrity verification, use the value D24F10DEB7A1D60EE381E2AEA8789117D0980FCC.

To purchase a license key for Rotor-Gene AssayManager v2.1.1:
    1. After purchasing Rotor-Gene AssayManager v2.1.1 software and licenses, click the "Download" button and follow the onscreen instructions to install. After successful installation of the Rotor-Gene AssayManager v2.1.1 and a plug-in, a “key file” will be generated. This "key file" will be attached to the "Technical Support Form" in Step 5, below.   
    2. Go to the Technical Support Form. Make sure to include all required information and your Rotor-Gene Q or Rotor-Gene Q MDx serial number when filling out the form.       
    3. For Question 2, "Type of Request", select “Other”.
    4. For Question 4, “Your inquiry”, enter "Rotor-Gene AssayManager license request” and include your purchase order number, from your order confirmation.
    5. Include the "key file" from Step 1 as an attachment.
    6. Submit the form. QIAGEN Technical Services will respond via email with a license file within 1–2 working days.    
    7. After receiving your computer-specific Rotor-Gene AssayManager v2.1.1 license file, archive the license file locally on your computer.

IMPORTANT: The license file must always exist on the computer that has Rotor-Gene AssayManager v2.1.1 installed. The license file location should be protected and not subject to change.





试剂盒操作手册 (1)
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)