MagAttract Viral RNA M48 Kit
从多达300 µl的血清或血浆样本中自动纯化病毒RNA
Products
MagAttract Viral RNA M48 Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
MagAttract Viral RNA Kit (960)
Cat. No. / ID: 955538
Automated virus preparation on a third-party platform (KingFisher Flex). Proteinase K and buffers for offboard lysis, MagAttract Suspension G beads and buffers for onboard bind, wash and elute steps.
MagAttract Viral RNA Kit (960) 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。
特点
- 快速纯化高品质的病毒RNA
- 每批可灵活处理6–48个样本
- 易于优化RNA的浓度
产品详情
MagAttract Viral RNA M48 Kit应用经验证的MagAttract磁珠技术，在BioRobot M48工作站上自动纯化病毒RNA。灵活的洗脱体积，易于优化病毒RNA的浓度，适合各种下游应用。在移液吸头中进行病毒RNA的纯化和磁力分离，提高了整个流程的效率。
绩效
MagAttract Viral RNA M48 Kit纯化一个样本获得的病毒RNA的量通常小于1 µg ，因而采用分光光度计很难定量。推荐使用定量扩增的方法确定RNA的产量。
原理
MagAttract Viral RNA M48 Kit用于从血清和血浆中全自动纯化病毒RNA。MagAttract技术配合硅胶膜技术快速、高效的纯化RNA，硅胶膜覆盖在方便使用的磁珠表面。样本放在1.5 ml或2 ml的样品管中，在BioRobot M48工作站上处理。每批可处理6–48个样本，样本来源为至多300 µl的血清或血浆。纯化获得的RNA的量取决于样本类型和病毒滴度。
程序
MagAttract Viral RNA M48流程中，病毒RNA在离液盐中结合到含有磁珠的硅胶膜表面。随后，结合到磁珠上的病毒RNA使用2种不同的洗涤缓冲液有效洗涤。然后，使用不含RNase的洗脱液，在65ºC条件下，将高品质病毒RNA洗脱。
应用
MagAttract Viral RNA M48 Kit和BioRobot M48工作站共同使用，纯化得到的高品质RNA适用于各种下游应用，包括：
- 病毒流行病学和基因分型
- 传染性疾病的研究
辅助数据和图表
Recovery of SARS-CoV-2 RNA from respiratory swab supernatants.
RNA purified from respiratory swab supernatant using the indicated kits was subjected to RT-PCR (mean value and deviation of threshold cycle shown). (A) negative samples spiked with AccuPlex SARS-CoV-2; (B) 1:10 diluted SARS-CoV-2 positive samples.
Specifications
| Features | Specifications |
|---|---|
| ApplicationsZH | PCR, real-time, genotyping, sequencing |
| Sample amount | 300 µl |
| For automated processing | BioRobot M48 Workstation |
| Elution volume | 50–100 µl |
| Main sample type | Serum, plasma |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Viral RNA |
| Processing | Automated |
| Format | Filter tip (up to 48 per run) |
| Technology | Magnetic particles |
资源
快速启动实验方案 (1)
试剂盒操作手册 (1)
安全数据表 (1)
Certificates of Analysis (1)
FAQ
Where can I download the protocol for the KingFisher Flex?
Who can help if it is not clear if issues are caused by the third party instrument or by the chemistry?
Who can help in case of issues with the third party instrument?
Is the virus inactivated during the procedure?
I would like to use the kit on another third party instrument. Who should I contact?
Can I use sample volumes other than 300 µl?
Are protocols also available for other instruments (e.g., Hamilton Star and Tecan Fluent)?