QIAsymphony DSP Virus/Pathogen Kit

适用于1–96个样本的病毒核酸或细菌/病原体DNA自动化纯化

Products

QIAsymphony DSP Virus/Pathogen Kits可用于体外诊断。

QIAsymphony DSP Virus/Pathogen Midi Kit

Cat. No. / ID: 937055

For 96 preps (1000 µl each): includes 2 reagent cartridges and enzyme racks and accessories

QIAsymphony DSP Virus/Pathogen Mini Kit

Cat. No. / ID: 937036

For 192 preps (200 µl each): includes 2 reagent cartridges and enzyme racks and accessories

特点

标准化操作流程带来的结果高度可重复性
附带的条形码扫描功能可用于样本和试剂的全程追踪
经优化的实验步骤可获得高产量的纯化核酸
方便易用的创新性预装试剂条

产品详情

QIAsymphony DSP Virus/Pathogen Kits系列试剂盒配套QIAsymphony SP仪器可为体外诊断实验所需并来源于多种样本的病毒核酸和细菌/病原体DNA提供自动化纯化方案。该类试剂盒可分为小量和中量提取两种规格，适用于体积从200 µl到1000 µl的起始样本。对于组织、FFPE组织、培养的细胞及细菌样本，手工操作的样本预处理过程是必要的。经优化的一系列操作步骤可帮助从样本中获得较高产量的纯化DNA，灵活的洗脱体积为下游多种应用所需的不同DNA浓度优化提供了可能。创新的预装试剂条则能够最大程度的减少了实验设置和手工操作的时间。附带的条形码扫描功能可用于试剂的全程追踪。

原理

QIAsymphony DSP Virus/Pathogen Kits系列试剂盒可用于来源于血清、血浆或是脑脊液中的病毒核酸纯化，也适用于如呼吸道相关拭子、吸液、痰液以及灌洗液（BAL）或是尿液、泌尿拭子（宫颈和尿道）样本中的病毒核酸或细菌DNA纯化。该试剂盒还可用于从多种DNA、RNA病毒以及革兰氏阴性、阳性菌中进行核酸的纯化。

QIAsymphony DSP Virus/Pathogen Kits系列试剂盒将病毒或细菌核酸提取过程中硅胶膜法所具有的快速、高效优势同磁珠法所具有的操作便捷性进行了很好的结合（参见）。创新的即用型试剂预装条里已预装了纯化步骤所需的全部试剂，并且会由QIAsymphony SP仪器进行自动开封，最大程度地较少了环境污染的风险（参见）。经优化的实验操作步骤在获取高质量核酸的基础上还能高效的去除蛋白、核酸酶以及其他杂质。经QIAsymphony DSP Virus/Pathogen Kits系列试剂盒纯化得到的核酸可直接适用于下游多种应用（如PCR）。该试剂盒可灵活选择不同的洗脱体积。

查看图表

Schematic of the QIAsymphony SP principle

Prefilled, sealed reagent cartridges

程序

该纯化步骤是为确保对潜在可感染样本进行处理时的安全性和可重复性而设计的，分为4步：裂解、吸附、洗涤以及洗脱（参见 QIAsymphony DSP Virus/Pathogen 提取步骤）。工作台可进行快速设置，可节省宝贵的时间。实验时只需简单的将来自不同或相同试剂盒的最多2个试剂条放置于试剂抽屉中即可，在一次仪器运行过程中对于最多96个样本可同时运行多种不同的纯化步骤。相关试剂会根据样本量进行配套使用，不会造成试剂浪费而达到最大程度的成本控制。

采用 QIAsymphony DSP Virus/Pathogen Kit 的实验方案
实验方案	试剂盒	样本材料	处理体积
无细胞体液200	QIAsymphony DSP Virus/Pathogen Mini Kit	血浆、血清和 CSF	200 µl
无细胞体液500	QIAsymphony DSP Virus/Pathogen Midi Kit	血浆、血清和 CSF	500 µl
无细胞体液1000	QIAsymphony DSP Virus/Pathogen Midi Kit	血浆、血清和 CSF	1000 µl
复杂混合样本200	QIAsymphony DSP Virus/Pathogen Mini Kit	呼吸道样本（灌洗液、干拭子、传输介质、呼出物、痰）和泌尿生殖系统的样品（尿液、传输介质）	200 µl
复杂混合样本400	QIAsymphony DSP Virus/Pathogen Midi Kit	呼吸道样本（灌洗液、干拭子、传输介质、呼出物、痰）和泌尿生殖系统的样品（尿液、传输介质）	400 µl
复杂混合样本800	QIAsymphony DSP Virus/Pathogen Midi Kit	呼吸道样本（灌洗液、干拭子、传输介质、呼出物、痰）和泌尿生殖系统的样品（尿液、传输介质）	800 µl

查看图表

QIAsymphony DSP Virus/Pathogen procedure

应用

QIAsymphony DSP Virus/Pathogen Kits系列试剂盒纯化得到的高品质核酸可用于DNA、RNA病毒以及细菌病原体的灵敏检测。得到的产量是线性均一的，无论对于高滴度还是低滴度的病毒或是细菌都可以进行准确的定量分析。高品质的纯化核酸可直接适用于下游的所有应用，如测序或是诸如实时定量PCR、RT-PCR等较为灵敏的检测实验。

辅助数据和图表

QIAsymphony DSP Virus/Pathogen procedure

资源

For use with software version 4.0

Download Safety Data Sheets for QIAGEN product components.

For use with the QIAsymphony SP/AS instruments (software version 4.0)

For use with software version 4.0

FAQ

Tube carriers for up to 24 tubes (primary or secondary tubes or tubes containing internal controls) can be used. A list of compatible labware formats for sample input can be found at www.qiagen.com/goto/dsphandbooks. Slots 1–4 are used for samples to be processed. The internal control-carrier RNA-Buffer AVE mixture is loaded into Slot 5.

FAQ ID -2936

The ACS specifies the combination of a protocol and a predefined carrier RNA–internal control mixture. The user can define an internal control mixture using the QIAsymphony Management Console. A predefined internal control-carrier RNA-Buffer AVE mixture can be automatically identified by the instrument using a bar code label or it can be chosen manually.

FAQ ID -2933

Tissue: If no information about the expected yield is available, we recommend starting with 25 mg sample material. Depending on the yield obtained, the sample size can be increased in subsequent preparations to a maximum of 50 mg.

FFPE: Up to 4 sections, each with a thickness of up to 10 µm, or 8 sections with a thickness of up to 5 µm and a surface area of up to 250 mm2, can be combined in one preparation.

FAQ ID -2927

We recommend using racked Elution Microtubes CL (cat. no. 19588) for elution. Alternatively, 2 ml Sarstedt tubes (with screw caps, Sarstedt cat. no. 72.693) can be used. A list of compatible labware formats for eluates can be found at www.qiagen.com/goto/dsphandbooks.

FAQ ID -2937

The QIAsymphony DSP Virus/Pathogen Mini Kit is used with the Cellfree200, Complex200 and Complex200_OBL protocols.

The QIAsymphony DSP Virus/Pathogen Midi Kit is used with the Cellfree500, Cellfree1000, Complex400, Complex400_OBL, Complex800, and Complex800_OBL protocols.

All complex protocols require Buffer ATL, which is not included in the QIAsymphony DSP Virus/Pathogen Kits. Buffer ATL can be ordered separately (Buffer ATL (4 x 50 ml), cat. no. 939016).

FAQ ID -2930

Yes. The QIAsymphony DSP Virus/Pathogen Kits are designated “For In Vitro Diagnostic Use”. It is the user’s responsibility to validate system performance for any procedures used in their laboratory that are not covered by the QIAGEN performance evaluation studies. The system performance has been established in performance evaluation studies purifying viral DNA and RNA from human serum, plasma, or cerebrospinal fluid (CSF), and purifying viral DNA and RNA, as well as bacterial DNA, from respiratory and urogenital samples. To minimize the risk of a negative impact on the diagnostic results, adequate controls for downstream applications should be used. For further validation, the guidelines of the International Conference on Harmonisation of Technical Requirements (ICH) in ICH Q2 R(1) Validation of Analytical Procedures: Text and Methodology are recommended. Any diagnostic results that are generated must be interpreted in conjunction with other clinical or laboratory findings.

FAQ ID -2939

Plasma, serum, and CSF samples can be processed using the QIAsymphony DSP Virus/Pathogen cellfree protocols. Respiratory samples (sputum, BAL, aspirates, dried swabs, transport media), and urogenital samples (urine, urogenital swabs, transport media) can be processed using the pathogen complex protocols.

FAQ ID -2928

For all cellfree and complex protocols, an eluate volume of 60 µl, 85 µl, or 110 µl can be selected. These are the minimum accessible volumes of eluate in the final elution tube. The initial elution volumes, which have to be used for the internal control, as well as titer calculations, are 90 µl, 115 µl, and 140 µl.

FAQ ID -2938

Pretreatment can improve the nucleic acid extraction for some sample types. Viscous samples (e.g., sputum, respiratory aspirates) might require liquefaction to enable pipetting. Performance may be improved for Gram positive bacteria by pretreatment with lysozyme. Pretreatment protocols are provided in the QIAsymphony DSP Virus/Pathogen Handbook.

FAQ ID -2931

The QIAsymphony DSP Virus/Pathogen Mini kit can be used to process 192 samples (96 per reagent cartridge). The Midi kit can be used to process 96 samples (48 per reagent cartridge) using the Cellfree1000, Complex800, and the Complex800_OBL protocols. The Midi Kit can also be used to process 144 samples (72 per reagent cartridge) using the Cellfree500, Complex400, and the Complex400_OBL protocols.

FAQ ID -2935

The sample volume depends on the QIAsymphony DSP Virus/Pathogen protocols:

Cellfree200 — 200 µl
Cellfree500 — 500 µl
Cellfree1000 — 1000 µl
Complex200 and Complex200_OBL — 200 µl
Complex400 and Complex400_OBL — 400 µl
Complex800 and Complex800_OBL — 800 µl

FAQ ID -2929

Sample material can be stored at 2–8°C for up to 6 h. For longer storage, samples should be kept at –20°C or –70°C.

FAQ ID -2934

No. The use of internal controls is not mandatory, but is strongly recommended. When using internal controls, they are added to the carrier RNA mixture (total volume of the mixture must be 120 µl).

FAQ ID -2932