FlexiGene DNA Kit

在单管中，从全血、白膜层和培养细胞中纯化基因组DNA

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FlexiGene DNA Kit (250)

Cat. No. / ID: 51206

For isolation of genomic DNA from whole blood, buffy coat, and cultured cells in a single tube with fast kit preparation

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FlexiGene DNA Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

容易处理，无样本混淆的风险
可从0.1–20 ml全血中纯化
可从全血、白膜层和培养细胞中直接纯化
纯化速度快
无需有机提取

Product Details

FlexiGene体系可通过简单的离心操作从人全血、白膜层和培养细胞中分离高纯度的DNA。FlexiGene DNA Kit包括缓冲液和QIAGEN蛋白酶，可直接在单管中简单的进行纯化。联系Autogen, Inc.，咨询FlexiGene试剂的自动纯化解决方案。

Performance

使用FlexiGene体系纯化的DNA可达150 kb（参见" High-quality genomic DNA"）。可用于扩增反应和Southern印迹实验，也可存档（参见" Efficient downstream PCR analysis"）。

DNA在单管中通过离心操作，从0.1 – 20 ml（血液）、0.1 – 2 ml（白膜层）或1 – 2 x 10⁶个细胞（培养细胞）中纯化DNA。1小时内处理12个样本。依据样本大小，再水合体积0.2 – 1 ml。

使用FlexiGene系统从不同类型的样本中纯化获得的DNA产量

样品体积
DNA 产量（µg）

血液*
0.3 ml

11–14

血液*
2.0 ml

75–90

血液*
10.0 ml

380–460

白膜层
0.5 ml

110–130

HeLa细胞
10⁶ cells

25–35

* 全血以正常的白细胞计数（7x10⁶ 个细胞/ml）。

使用FlexiGene系统从不同类型的样本中纯化获得的DNA产量
样品	体积	DNA 产量（µg）
血液*	0.3 ml	11–14
血液*	2.0 ml	75–90
血液*	10.0 ml	380–460
白膜层	0.5 ml	110–130
HeLa细胞	10⁶ cells	25–35

See figures

High-quality genomic DNA.

Efficient downstream PCR analysis.

Principle

FlexiGene DNA体系可从不同量的全血、白膜层和培养细胞中纯化基因组DNA。纯化过程在单管中进行，费用少，操作简单。纯化的DNA在多种下游应用中表现良好。

Procedure

首先在样本中加入裂解液（参见""）。细胞核和线粒体离心沉于底部，使用包含QIAGEN Protease的变性缓冲液重新悬浮。然后蛋白裂解，加入异丙醇沉淀DNA，离心处理，70%乙醇洗涤和干燥。使用水合缓冲液重新悬浮的DNA可即用于下游分析或–20°C贮存。可从全血、白膜层和培养细胞中获得高产量的DNA。

See figures

FlexiGene DNA procedure.

Applications

FlexiGene DNA Kit纯化的DNA可用于多种下游应用，包括：

PCR
Southern印迹
存档

Supporting data and figures

FlexiGene DNA procedure.

The first step is addition of lysis buffer to the samples. Cell nuclei and mitochondria are pelleted by centrifugation and resuspended in denaturation buffer containing QIAGEN Protease. Following protein digestion, DNA is precipitated by addition of isopropanol, recovered by centrifugation, washed in 70% ethanol, and dried. DNA is resuspended in hydration buffer and is ready for direct use in downstream assays or storage at –20ºC.

Specifications

Features	Specifications
Applications	PCR techniques, restriction digest, Southern blotting, sequencing
Sample amount	100 µl - 20 ml
Format	Scalable
Time per run or per prep	25 - 45 minutes (+ DNA rehydration)
Main sample type	Whole blood, buffy coat, cultured cells
Technology	Single tube precipitation method
Processing	Manual
Yield	35 µg / ml whole blood
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Genomic DNA
Elution volume	200 µl - 1000 µl

Resources

For purification of DNA from human whole blood, buffy coat, cultured cells

This protocol is designed for purification of DNA from up to 10 ml clotted blood using the FlexiGene DNA Kit.

7 years storage at 2-8°C and -20°C

Download Safety Data Sheets for QIAGEN product components.

For purification of DNA from human whole blood, buffy coat, cultured cells

Download Safety Data Sheets for QIAGEN product components.

7 years storage at 2-8°C and -20°C

This protocol is designed for purification of DNA from up to 10 ml clotted blood using the FlexiGene DNA Kit.

Publications

The (TAAAA)n microsatellite polymorphism in the SHBG gene influences serum SHBG levels in women with polycystic ovary syndrome.

Ferk P; Teran N; Gersak K;

Hum Reprod; 2006; 22 (4):1031-6 2006 Dec 22 PMID:17189294

A functional SNP of interferon-gamma gene is important for interferon-alpha-induced and spontaneous recovery from hepatitis C virus infection.

Huang Y; Yang H; Borg BB; Su X; Rhodes SL; Yang K; Tong X; Tang G; Howell CD; Rosen HR; Thio CL; Thomas DL; Alter HJ; Sapp RK; Liang TJ;

Proc Natl Acad Sci U S A; 2007; 104 (3):985-90 2007 Jan 10 PMID:17215375

Lipopolysaccharide-activated CD4+CD25+ T regulatory cells inhibit neutrophil function and promote their apoptosis and death.

Lewkowicz P; Lewkowicz N; Sasiak A; Tchórzewski H;

J Immunol; 2006; 177 (10):7155-63 2006 Nov 15 PMID:17082633

Predictors of vitamin B6 and folate concentrations in older persons: the InCHIANTI study.

Gori AM; Sofi F; Corsi AM; Gazzini A; Sestini I; Lauretani F; Bandinelli S; Gensini GF; Ferrucci L; Abbate R;

Clin Chem; 2006; 52 (7):1318-24 2006 May 11 PMID:16690736

Past adult lead exposure is linked to neurodegeneration measured by brain MRI.

Stewart WF; Schwartz BS; Davatzikos C; Shen D; Liu D; Wu X; Todd AC; Shi W; Bassett S; Youssem D;

Neurology; 2006; 66 (10):1476-84 2006 May 23 PMID:16717205

FAQ

Yes, please follow the User-Developed Protocol 'Purification of DNA from clotted blood using the FlexiGene DNA Kit' (FG01).

FAQ ID -973

Yes. We have shown that DNA purified with the Flexigene DNA Kit is stable for at least 7 years when being stored at either 2-8°C or -20°C. However, the DNA stability is dependent upon storage conditions. The data is available here under Additional Resources.

FAQ ID -1317

Yes, we have the following protocols:

Isolation of genomic DNA from frozen clotted whole blood using the QIAGEN Genomic-tip 100/G (QG02). TEST

You will need to prepare the required buffers according to the recipes in Appendix A of the QIAGEN Genomic DNA Handbook, or you can purchase the Genomic DNA Buffer Set containing pre-made solutions. Alternatively, the QIAGEN Blood & Cell Culture Midi Kit, containing Genomic-tips 100/G and buffers, can be used.

Purification of archive-quality DNA from clotted whole blood using Clotspin Baskets and the Gentra Puregene Blood Kit (PG03).
Purification of archive-quality DNA from clotted whole blood using the Gentra Puregene Tissue Kit or Gentra Puregene Mouse Tail Kit (PG04).
Purification of DNA from clotted blood using the FlexiGene DNA Kit (FG01).

FAQ ID -900. Test

Buffer FG3, the hydration buffer supplied in the FlexiGene DNA Kit, is a 10 mM Tris-Cl solution at pH 8.5.

FAQ ID -356

FlexiGene DNA Kit

在单管中，从全血、白膜层和培养细胞中纯化基因组DNA

Features

Product Details

Performance

使用FlexiGene系统从不同类型的样本中纯化获得的DNA产量 样品 体积 DNA 产量（µg） 血液* 0.3 ml 11–14 血液* 2.0 ml 75–90 血液* 10.0 ml 380–460 白膜层 0.5 ml 110–130 HeLa细胞 106 cells 25–35 * 全血以正常的白细胞计数（7x106 个细胞/ml）。

See figures

Principle

Procedure

See figures

Applications

Supporting data and figures

FlexiGene DNA procedure.

Specifications

Resources

Publications

The (TAAAA)n microsatellite polymorphism in the SHBG gene influences serum SHBG levels in women with polycystic ovary syndrome.

A functional SNP of interferon-gamma gene is important for interferon-alpha-induced and spontaneous recovery from hepatitis C virus infection.

Lipopolysaccharide-activated CD4+CD25+ T regulatory cells inhibit neutrophil function and promote their apoptosis and death.

Predictors of vitamin B6 and folate concentrations in older persons: the InCHIANTI study.

Past adult lead exposure is linked to neurodegeneration measured by brain MRI.

FAQ