QIAamp 96 DNA QIAcube HT Kit

用于从血液、细胞和组织中自动、高通量提取总DNA

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QIAamp 96 DNA QIAcube HT Kit (5)

Cat. No. / ID: 51331

For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers

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KitAccessories

QIAamp 96 DNA QIAcube HT Kit

QIAcube HT Plasticware

QIAamp 96 DNA QIAcube HT Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

简单、可靠的自动化处理，省时且经济
适于多种类型样本的标准化方法
特殊样本也具有高产率
纯化得到的高品质DNA适用于多种下游应用
具有针对多样的起始材料进行了优化的实验方案

Product Details

QIAamp 96 DNA QIAcube HT Kit可从血液、细胞和组织样本便利的自动提取总DNA（如基因组、线粒体和病原体DNA）。其使用成熟的QIAamp硅胶膜技术，以96孔板形式纯化，方便地去除污染物和抑制剂，获得可直接用于下游分析的优质病原体核酸。

Performance

QIAamp 96 DNA QIAcube HT Kit可以96孔板的形式快速、可靠地自动提取总DNA。其所获得的结果具有高度可重复性，孔间的DNA回收率无显著差异（参见）。

使用QIAamp 96 DNA QIAcube HT Kit从血液样本中自动抽提总DNA的过程简单、快速且可靠。可处理含有常规抗凝剂的新鲜或冰冻的全血样本，所获得的DNA产物与QIAGEN其他的纯化方案具有同等的质量水平（参见和）。

QIAcube HT与专用的QIAamp 96 DNA QIAcube HT Kit使得用户能在增加样本纯化通量的同时，无需担心产物质量或可靠性下降的风险。该方法能够提供高产率、高纯度的DNA，并和其他的QIAGEN DNA纯化解决方案一样，十分适合于下游的分析应用（参见和）。

See figures

Reproducible 96-well DNA purification.

Agarose gel of purified genomic DNA.

Analysis of genomic DNA purified from human blood.

High performance in downstream assays.

Purification of genomic DNA from tissues.

Principle

QIAamp 96 DNA QIAcube HT Kit能够从包括新鲜或冰冻组织、细胞和血液在内的多种来源样本自动纯化总DNA（例如基因组、线粒体和病原体DNA）。采用柠檬酸、肝素或EDTA等常规抗凝剂处理过的样品都可使用该试剂盒。此操作能够制备优质DNA产物，十分适用于下游的PCR及其他酶促反应。

QIAamp 96 DNA QIAcube HT Kit整合了硅胶膜的选择性结合能力与96孔板的高通量处理能力，更具备全自动设计，能在QIAcube HT仪器上同时处理24–96份样本。

Procedure

在QIAcube HT仪器上使用QIAamp 96 DNA QIAcube HT操作步骤迅速、简便。首先使用QIAGEN Proteinase K对样本进行裂解。调节缓冲条件以提供最优的DNA结合条件，并将裂解产物载入QIAamp 96孔板。抽真空后，DNA会选择性地与QIAamp膜相结合，而污染物则被洗去。通过三次高效的洗涤步骤能够去除残存的污染物和酶抑制剂。最后在真空条件下，在200 µl AE缓冲液中洗脱纯净的DNA。

Applications

QIAamp 96 DNA QIAcube HT Kit能够生成优质DNA，适用于多种下游分析应用，包括如下领域之中的各种应用：

生命科学研究
禽畜养殖
谱系基因分型检测
兽医病原体研究
常规检测

Supporting data and figures

Agarose gel of purified genomic DNA.

Genomic DNA was purified from 50, 100, 150, and 200 µl human blood with QIAcube HT and the QIAamp 96 DNA QIAcube HT Kit. Purified DNA was eluted in 200 µl, and 7 µl of each eluate was analyzed on a 0.8% agarose gel in 1x TBE. M: Lambda HindIII marker.

Resources

Interim version of QIAamp 96 PowerFecal protocol to be used with QIAamp 96 PowerFecal QIAcube HT Kit.

Q Protocol notes for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit; use with the QIAamp 96 DNA QIAcube HT V1.qsp run file. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.

Interim version of QIAamp 96 Virus protocol to be used with QIAamp 96 Virus QIAcube HT Kit.

Interim version of QIAamp 96 DNA protocol to be used with QIAamp 96 DNA QIAcube HT Kit.

Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.

Handbook for purification of genomic DNA from blood, cells, and soft tissue on the QIAcube HT using the QIAamp 96 DNA QIAcube HT Kit. For use with QIAcube HT Operating Software 4.17; not compatible with QIAcube HT Prep Manager Software.

For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17

For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software

For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17

For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software

Download Safety Data Sheets for QIAGEN product components.

For fast and reliable automated 96-well nucleic acid purification

Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Prep Manager Software v1.1.1.

Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software

For fast and reliable automated 96-well nucleic acid purification

Download Safety Data Sheets for QIAGEN product components.

For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17

For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software

Installation file (.msi) for installing the Q Protocol run file (.qsp) in the QIAcube HT software. For use with QIAcube HT Operating Software 4.17; not for use with QIAcube HT Prep Manager Software.

Instrument user manual for automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology; for use with QIAcube HT Prep Manager Software v1.1.1.

FAQ

No, the two software applications are not interchangeable.

Additionally, the QIAcube HT and QIAxtractor software cannot be installed on the same computer. The QIAcube HT software versions is v4.17 or higher, while the QIAxtractor software version is v4.16 or lower.

FAQ ID -9102

No, this upgrade is not possible.

FAQ ID -9104

The Support Packages are important when reporting an issue to QIAGEN Technical Services. Upon completion of each run, the software automatically saves a support package.

To retrieve a support package, click Help in the QIAcube HT or QIAxtractor software and select Choose support package. In the new windows, select the support package that corresponds to the issue and send it to QIAGEN Technical Services.

FAQ ID -9100

1. Check the vacuum tubing to make sure it is not pinched or obstructed in some way. Sometimes the tubing can become pinched against the bench top when the vacuum station is placed too far from the instrument.

2. Make sure that the channeling adapter is properly placed. Check if the carriage is moving the plate completely from the waste chamber position to the elution chamber position. If the calibration seems incorrect, the instrument may need to be re-taught at this position.

3. When using less than a full plate, make sure all the unused columns are covered with an adhesive seal.

Also, make sure each column of 8 samples is full. When processing a number of samples that results in < 8 samples per column, use water as a substitute in the empty positions until the column is filled. This ensures even vacuum across all unsealed well positions.

4. Make sure correct plates are used for the selected protocol.

5. When the vacuum is on, please listen for any hissing noise from the tubing that is coming down to the waste container. This could indicate leaks in the tubing.

If the issue persists, please contact QIAGEN Technical Service.

FAQ ID -9106

Make sure the QIAcube HT or QIAxtractor software uses the same COM port number as assigned to the QIAcube HT or QIAxtractor instrument on the computer. The COM port setting can be checked by clicking Options\Robot Setup\Select COM port in the QIAcube HT or QIAxtractor software.

Ensure that the cable between the instrument and the computer is securely inserted. Power on the instrument first, and then launch the QIAxtractor/QIAcube HT software.

The instrument has both RS-232 (serial) and USB interfaces for computer connection. Try an RS-232 cable if the communication cannot be established between the instrument and computer with a USB cable.

If the issue persists, please contact QIAGEN Technical Services.

FAQ ID -9101