QIAamp 96 DNA Swab BioRobot Kit

从拭子样本中高通量自动纯化DNA

S_1084_5_GEN_disclaimer

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QIAamp 96 DNA Swab BioRobot Kit (12)

Cat. No. / ID:   965842

For 12 x 96 DNA preps: 12 QIAamp 96 Plates, Buffers, QIAGEN Proteinase K, AirPore Tape Sheets, Tape Pad, S-Blocks, Racks with Collection Microtubes (1.2 ml), Caps
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QIAamp 96 DNA Swab BioRobot Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的(再)订购

特点

  • 快速纯化获得高品质DNA
  • 无需有机提取或乙醇沉淀
  • 重复性好,产量高
  • 完全去除污染物和抑制剂

产品详情

QIAamp 96 DNA Swab BioRobot Kit使用经验证的QIAamp硅胶模技术在BioRobot Universal System上使用,2.5个小时内可纯化多达192个拭子样本。经优化的操作方法适用于风干的棉签拭子或DACRON拭子,也可用于其他拭子样本。

绩效

经验证的QIAamp 96孔板技术提供DNA回收和纯化的孔间一致性,并且样品之间无交叉污染(参见" Cross-contamination test")。有效地去除酶抑制剂,确保在下游应用中的可靠性能,如:TaqMan、LightCycler和iCycler荧光PCR的表现(参见" Reliable performance")。

纯化的DNA可即用于分子诊断和法医学的各种应用:

  • 遗传学疾病检测,如血色沉着病或凝血因子V基因
  • 身份鉴定,如HLA分型
  • 亲子鉴定(参见"Multiplex STR analysis"和"GeneScan analysis")
查看图表

原理

不需使用苯酚-氯仿抽提。DNA特异性与QIAamp硅胶膜结合,污染物流走。PCR抑制剂,如:二价阳离子和蛋白,可通过两步有效的洗涤步骤被完全去除,结合在离心柱上的纯DNA可用水或试剂盒中的缓冲液洗脱。使用QIAamp技术从拭子中获得的DNA可即用于PCR和印迹实验中。

程序

QIAamp 96 DNA Swab BioRobot Kit使用快速、自动96孔板式操作简化了从拭子中分离DNA的过程(参见" QIAamp 96 DNA Swab BioRobot procedure")。经优化的操作方法适用于风干的棉签拭子或DACRON拭子,也可用于其他拭子样本也。使用QIAamp 96 DNA Swab BioRobot Kit,每个拭子(口腔拭子)可得到1–2 µg的DNA,洗脱体积为150 µl。

QIAamp样品制备技术是完全经过验证的。

查看图表

应用

QIAamp 96 DNA Swab BioRobot Kit利用QIAamp技术,以96孔板规格便利的进行高通量纯化。可在2个小时内从多达96个拭子样本中纯化DNA。样本类型包括:

  • 口腔拭子
  • 咽拭子
  • 眼拭子

辅助数据和图表

Specifications

FeaturesSpecifications
ApplicationsZHPCR, blotting
Yield1–2 µg per swab
Format96-well plate
For automated processingBioRobot Universal System, BioRobot Genotyping, BioRobot 9604
Sample amount1 swab per well
ProcessingAutomated
Elution volume150 µl
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA
Main sample typeSwabs
TechnologySilica technology
Time per run or per prep<2 hours

资源

试剂盒操作手册 (1)
For automated purification of genomic DNA from buccal swabs using the BioRobot Universal System, BioRobot Genotyping, or BioRobot 9604
安全数据表 (1)
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)

FAQ

What is the difference between QIAGEN Protease and QIAGEN Proteinase K provided in various QIAamp Kits?

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12