QIAamp Media MDx Kit

从液体培养基样本中自动纯化DNA

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QIAamp Media MDx Kit (12)

Cat. No. / ID: 965752

For 12 x 96 preps: 12 QIAamp 96 Plates, Buffers, Proteinase K, S-Blocks, Disposable Troughs, Racks with Elution Microtubes CL (0.4 ml), Carrier RNA, Top Elute Fluid, Caps, Tape Pad

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QIAamp Media MDx Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

从各种液体培养基样本中纯化DNA
全自动化操作流程，无需离心步骤
纯化病毒和其他DNA
有效去除污染物，获得高品质DNA

Product Details

QIAamp Media MDx Kit应用全自动操作流程，从液体培养基，如子宫拭子运输培养基中纯化DNA。在BioRobot MDx全自动核酸分离纯化系统上全自动化处理96个样本仅需225分钟，包括条形码输入，装载检查和全过程记录，整个过程无需人工操作。

Performance

纯化的核酸不含蛋白、核酸酶和其他污染物。高重复性，确保下游分析中获得准确的结果（参见"Highly reproducible yields"）。获得的高品质核酸可即用于各种下游分析，如real-time PCR（参见"Linear yields of high-quality nucleic acids"）。

Principle

QIAamp Media MDx Kit应用成熟的技术从液体培养基中纯化核酸。该试剂盒采用具有选择性结合特性的硅胶膜，配合高通量的96孔板模式，在BioRobot MDx工作站上全自动同时处理多达96个265 µl的样品。

Procedure

液体运输培养基能交联细胞，并使细胞聚集，进而难以裂解。QIAamp Media MDx Kit中经优化的缓冲液能有效地裂解这些样本、稳定核酸，并提高核酸对QIAamp膜的吸附选择性（参见"Protocol"）。在QIAamp 96孔板上加入乙醇和溶解产物。使用洗涤缓冲液去除杂质，包括乙醇和磷酸盐，然后用水或低盐缓冲液洗脱纯化的、可直接使用的DNA。正在申请专利的电脑控制真空技术，确保在液体细胞培养基中的不稳定污染物有效去除，如乙醇。

Applications

QIAamp Media MDx Kit可从多种来源的样本中纯化细胞、细菌和病毒核酸，包括：

含乙醇的液体细胞培养基（如：PreservCyt和SurePath）
磷酸缓冲液体运输培养基（如：M4RT）
尿液
干血斑、血卡和拭子

Supporting data and figures

QIAamp Media MDx protocol.

Linear yields of high-quality nucleic acids.

Specifications

Features	Specifications
Applications	PCR, real-time PCR
Elution volume	120 µl
Main sample type	Liquid media
Processing	Automated
Technology	Silica technology
Time per run or per prep	200 minutes (96 samples)
Sample amount	265 µl
Format	96-well plate
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	Viral DNA and RNA, bacterial DNA and RNA, cellular DNA and RNA
For automated processing	BioRobot MDx Workstation
Yield	Varies

Resources

For automated purification of nucleic acids from liquid media using the BioRobot MDx workstation

Download Safety Data Sheets for QIAGEN product components.

For automated purification of nucleic acids from liquid media using the BioRobot MDx workstation

FAQ

QIAGEN Proteinase K is a subtilisin-type protease, which cleaves at the carboxyl side of hydrophobic, aliphatic and aromatic amino acids. It is particularly suitable for short digestion times. It possesses a high specific activity over a wide range of temperatures and pH values with substantially increased activity at higher temperature. Soluble calcium is not essential for enzymatic activity. This means that EDTA, which is used to inhibit Mg2+-dependent enzymes such as nucleases, will not inhibit Proteinase K activity.

QIAGEN Protease is a broad-specificity Serine protease with high activity, cleaving preferentially at neutral and acidic residues. It is an economical alternative to Proteinase K for isolation of native DNA and RNA from a variety of samples.

For more information on activity of QIAGEN Protease and Proteinase K in various buffers please click here.

FAQ ID -761

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728