QIAxcel DNA Kits

使用QIAxcel体系全自动分析DNA片段

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QIAxcel DNA High Resolution Kit (1200)

Cat. No. / ID: 929002

QIAxcel DNA High Resolution Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips

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Cartridge type

High Resolution

Screening

Fast Analysis

High Sensitivity

QIAxcel DNA Kits 旨在用于分子生物学应用。这些产品不能用于疾病诊断、预防和治疗。

✓ 全天候自动处理在线订单

✓ 博学专业的产品和技术支持

✓ 快速可靠的（再）订购

特点

采用安全、操作简单的即用型预制胶
简化的工作流程，分析时间最短化
可检测到低至0.1 ng/µl的核酸，结果可靠
分辨率可达3–5 bp
标准化的处理过程，包括数字化数据采集

产品详情

配合QIAxcel体系，QIAxcel DNA Kits每批可全自动依据大小分离和定量多达96个样本DNA片段。QIAxcel技术基于使用凝胶卡夹的毛细管电泳，实现无可比拟的高分辨率、高速和高通量。QIAxcel凝胶卡夹可重复使用，每批12个样本可使用250次。仪器预装的处理方法适合大多数应用。QIAGEN也可提供用户需要的处理方法。

绩效

简单的高分辨率分析

生物基因分型、基于PCR的cDNA文库筛选以及许多生物医药研究和分子生物学应用都要求对PCR产物片段的大小有很高的分辨率。QIAxcel High Resolution Kit配合QIAxcel仪，可对15 bp–5 kb的DNA片段进行分析。分离小于500 bp的片段，分辨率达3–5 bp，其数据可靠性比传统的高分辨率琼脂糖凝胶电泳更高（参见" Sensitive detection of a mutated cancer-related gene"）。QIAxcel体系对于未经稀释的扩增反应中的DNA检测灵敏度达0.1 ng/µl。QIAxcel体系利用少量样本也可获得可靠的结果。

QIAxcel DNA Screening Kit非常适合限制性内切酶酶切产物、单重或多重定量PCR产物和其他应用产物DNA片段的常规分析（参见" High-resolution analysis of multiplex PCR samples"）。使用凝胶卡夹使工作流程更加高效，减少对大小在15 bp–3 kb之间的DNA片段进行分析的时间。处理96个样本在大约1小时内即可完成。对于小于1 kb的片段，其分辨率为20–50 bp。每次分析样本消耗量少于0.1 µl，为其他下游应用节约了珍惜样本。

QIAxcel DNA Fast Analysis Kit非常适合在单重或多重定量PCR产物应用中对DNA片段进行常规分析。使用凝胶卡夹使工作流程更加高效，减少对大小在15 bp–3 kb之间的DNA片段进行分析的时间。处理12个样本在大约3分钟内即可完成。处理96个样本在大约25分钟内即可完成（参见" Screenshot showing data from PCR fragment analysis"）。使用QIAxcel DNA Fast Analysis凝胶卡夹分辨率可达50 bp，分析样本消耗量少于0.1 µl。

查看图表

Sensitive detection of a mutated cancer-related gene.

High-resolution analysis of multiplex PCR samples.

Screenshot showing data from PCR fragment analysis.

原理

QIAxcel体系采用毛细管凝胶电泳技术，能够依据核酸的大小进行快速分离（参见" Sample separation process"）。不同于传统的琼脂糖凝胶电泳，分离是在含有即用型预制胶的毛细管中进行。样本自动上样到单个的毛细管中，然后加压。带负电的核酸通过毛细管移到正极。使用琼脂糖凝胶电泳时，小分子核酸比大分子核酸移动得快。而毛细管电泳，它们会通过一个检测和衡量荧光信号的检测器。光电倍增管器将荧光信号转为电子数据，然后传输到电脑上再使用QIAxcel ScreenGel软件进一步处理。处理后，可通过电泳图谱和胶图两种方式查看数据。

查看图表

Sample separation process.

程序

QIAxcel DNA Kits简化了分离和分析DNA的流程，且更加安全。只需简单的几个步骤即可：预制胶卡夹、预装缓冲液槽及装载有样本的96孔板或PCR管或联管、选择分离方法。无需冗长的制胶及用户培训，简化了日常工作的整个流程。运行开始几分钟内，会在电脑屏幕上实时出现第一个结果（参见" Reduced run times"）。

查看图表

Reduced run times.

应用

预设方法配合相应的凝胶卡夹，能够分离和分析多种核酸，包括单重或多重PCR片段、限制性内切酶消化的DNA和合成的寡聚核苷酸。

	QIAxcel DNA High Resolution Kit	QIAxcel DNA High Sensitivity Kit^§	QIAxcel DNA Screening Kit	QIAxcel DNA Fast Analysis Kit
Size range	15 bp – 20 kb; displaying up to 50 kb	15 bp – 3 kb	15 bp – 5 kb	15 bp – 3 kb
Resolution*	3–5 bp	20–50 bp	20–50 bp	50 bp
Limit of detection	0.1 ng/μl	5 pg/μl	0.1 ng/μl	0.1 ng/μl
Samples per cartridge	1200 samples	1200 samples	2400 samples	3000 samples
Run time^†	9–25 min	~9 min	~7 min	~3 min
Application area^‡	High-resolution genotyping VNTR, STRs, etc. gDNA integrity check NGS library check	Quality control of liquid biopsy extracts NGS library check	Fast PCR screening (single and multiplex) Restriction digest, plasmid gDNA integrity check NGS library check	Fast single amplicon analysis, e.g., HLA typing

STR: short tandem repeat; VNTR: variable number of tandem repeat.

* For 100–500 bp range.

^† For 12 samples.

^‡ Including, but not limited to.

^§ Only works in combination with the QIAxcel Connect System.

辅助数据和图表

Reduced run times.

Nucleic acid separation on the QIAxcel system using the QIAxcel DNA High Resolution Kit takes less than 10% of the time of conventional high-resolution agarose.

资源

A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher

For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments

The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.

Download Safety Data Sheets for QIAGEN product components.

For electrophoretic analysis of DNA samples using the QIAxcel Connect system

This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.

For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6

FAQ

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824

The QIAxcel DNA Fast Analysis Cartridge can only be run on the QIAxcel Fast Analysis System with the BioCalculator Fast Analysis software and the grey software key.

All other QIAxcel Cartridges can only be run on the QIAxcel System with the BioCalculator software and the blue software key.

FAQ ID -1828

The concentration of the DNA marker, pUC18/HaeIII, provided in the QIAxcel DNA Kits is 5ug/ml in 1XTE buffer solution. Customers can run their own DNA size marker sample with different concentration levels using the provided Methods (i.e. M500, H500, L500 and so on).

The recommended method for all basic applications with the QIAxcel System is the M500 method. The BioCalculator software also allows customers to choose their own Sample Injection Time in the Sequence table. The M500 method provides 5KV for 20 seconds for the electro-kinetic injection of lower concentrated DNA samples. If the Injection Time is doubled or tripled (40 or 60 sec), you can expect double or triple intensities (also peak heights).

FAQ ID -1841

For the BioCalculator software, the default data folder is:

C:\Program Files\QIAGEN\QIAxcel BioCalculator\Data.

For the ScreenGel software, the default data folders are:

C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Experiment (for a computer with Windows XP operation system) or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Experiment\ (for a computer with Windows 7 operating system).

Alternatively, you can more conveniently access the ScreenGel experiment data folder using the menu options File\Open data directory\Experiment within the ScreenGel software.

FAQ -9008

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray.
Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day.
Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.

FAQ ID -1823

The reference size marker table created by ScreenGel version 1.1 or earlier is incompatible with ScreenGel version 1.2. Re-analyze the size marker run in ScreenGel version 1.2 and create a new reference size marker table for future use.

FAQ -9009

When using the BioCalculator software, ensure the color of the software key matches the type of cartridge used. For example, the QIAxcel Fast Analysis cartridge requires the red software key, while the QIAxcel High Resolution cartridge requires the blue software key.

In addition, check that the communication between the computer and the instrument is established. Further, check that the smart key is inserted securely.

If the issue persists, contact QIAGEN Technical Services and provide the cartridge ID and lot number, which is printed on the packaging of QIAxcel cartridges.

FAQ -9004

When using the BioCalculator software, select Settings under File in the Instrument control panel window. In the new windows, click Check Filter in the new window and follow the on-screen prompts.

When using the ScreenGel software, log in to the ScreenGel software with an account with basic or advanced privilege. Select System Check under the Service environment. Select Filter Check and click Start.

Please send the filter test result to QIAGEN Technical Services for further assistance.

FAQ -9006

Incorrect storage can lead to QX alignment marker degradation. The QX alignment markers can be used for 50 runs or 3 days, whichever comes first. Opened QX alignment marker should be store at –20°C between uses.

FAQ -9003

Clogged capillaries and/or re-using the calibration marker are the most common causes of failed calibration. Perform “Removing gel from blocked capillaries”, as outlined in Appendix D in the QIAxcel DNA handbook. Use a new set of calibration marker for each new calibration procedure.

FAQ -9001

Please delete the 6 obsolete method files as outlines in the Important Note QIAxcel ScreenGel Software

FAQ -9010

When using the BioCalculator software, select Detector test under File in the Instrument control panel window. Perform the Detector test with and without a cartridge latched inside the QIAxcel. Feedback is not shown on the screen when you select the Detect test. Instead, the file named detector.asc is saved at C:\Program Files\QIAGEN\QIAxcel BioCalculator. Change the file name before performing another detector test.

Note: Older versions of BioCalculator software save the detector.asc at C:\Program Files\QIAxcel BioCalculator.

When using the ScreenGel software, log in to the ScreenGel with an account with basic or advanced privilege. Select System Check under the Service environment. Select Detector Test and click Start. The file is saved at C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting\Troubleshooting_xxxxxxxx (current date in the format of YYYYMMDD) for a computer with Windows XP operating system or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting for a computer with Windows 7 operating system. Alternatively, you can perform a screen capture for the Detector test result.

Please send the Detector test results to QIAGEN Technical Services for further assistance.

FAQ -9006