EZ1 RNA Tissue Mini Kit

自动纯化高品质总RNA

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EZ1&2 RNA Tissue Mini Kit (48)

Cat. No. / ID: 959034

For 48 preps: Reagent Cartridges (Tissue RNA), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer RLT, RNase-free DNase I, RNase-free water

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The name of this kit was changed from "EZ1 RNA Tissue Mini Kit" to "EZ1&2 RNA Tissue Mini Kit" to demonstrate that it is compatible with the new EZ2 Connect instruments as well as the EZ1 Advanced XL. No changes were made to the well-established kit technology.

EZ1 RNA Tissue Mini Kit 旨在用于分子生物学应用。该产品不能用于疾病诊断、预防和治疗。

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

纯化多达30 μg的总RNA
可靠地处理多达10 mg的标准组织样本
无RNase污染，结果可靠
洗脱体积可调
程序设置简单，插卡即用

Product Details

EZ1 RNA Cell Tissue Kit利用磁珠技术可从多达10 mg的标准组织样本中自动纯化总RNA，试剂盒包含所有需要的试剂和耗材。纯化试剂预先封填在试剂条中，以确保可快速、便利的加载至仪器中。EZ1 RNA Tissue Mini Kit可用在EZ1 Advanced XL、EZ1 Advanced或BioRobot EZ1工作站上。

Performance

使用EZ1 RNA Tissue Mini Kit纯化获得高产量、高品质总RNA的稳定性好、重复性高（参见" Efficient purification and reproducible yields"和" Consistent highly pure RNA"）。

See figures

Efficient purification and reproducible yields.

Consistent highly pure RNA.

Principle

使用EZ1 RNA Tissue Mini Kit进行自动纯化需使用EZ1 Advanced XL和EZ1 Advanced XL RNA Card，EZ1 Advanced和EZ1 Advanced RNA Card或BioRobot EZ1和EZ1 RNA Card。EZ1卡预装有纯化程序，在控制面板提供操作向导，并提供工作站的运行指令。

仪器使用硅胶膜磁珠技术，可在一次操作中对1–14个样本（EZ1 Advanced XL）或1–6个样本（EZ1 Advanced和BioRobot EZ1）进行纯化。预封装的试剂条和仪器的自动感控锁可降低污染的风险，避免RNases导致的核酸降解。自动纯化流程重复性好、产量线性契合度高，覆盖宽范围的起始样本量。该试剂盒可从各种不同的组织样本类型中纯化获得高品质的总RNA，适用于多种基因表达分析的应用。RNA的产量依赖于多种因素，包括：样本量、组织类型和纯化状态。

Procedure

组织首先放入Buffer RLT中裂解、匀浆。全自动EZ1 RNA纯化操作后，RNA被一步纯化。即使很少量的DNA也会干扰RNA的下游应用，所以采用完整的DNase 裂解步骤去除基因组DNA的污染。总RNA洗脱体积为200 µl、100 µl或50 µl，可直接用于各种下游应用。

Applications

EZ1 RNA Tissue Mini Kit纯化得到的高品质RNA适用于各种下游应用，如：

应用real-time RT-PCR技术进行基因表达分析
应用芯片技术进行基因表达分析
肿瘤学和病理学研究

Supporting data and figures

Efficient purification and reproducible yields.

Using the EZ1&2 RNA Tissue Mini procedure, total RNA was purified from 10 mg samples of rat kidney and liver and from 5 mg samples of rat pancreas, spleen, and lung. RNA was purified from 3 replicate samples of each tissue type. Yields of total RNA were determined by absorbance (A₂₆₀) with correction for background (A₃₂₀). CV kidney = 15%; CV liver = 1%; CV pancreas = 8%; CV spleen = 10%; CV lung = 22%.

Specifications

Features	Specifications
Applications	PCR, real-time RT-PCR, microarray
Time per run or per prep	40 minutes
Technology	Magnetic particles
Elution volume	50–200 µl
Processing	Automated
Format	Sample tube
Stabilization	Yes
For automated processing	BioRobot EZ1
Main sample type	Tissue samples
Sample amount	5–10 µg
Yield	<30 µg
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein	RNA

Resources

Fully automated low- to medium-throughput purification of nucleic acids

Download Safety Data Sheets for QIAGEN product components.

For purification of total RNA from tissue and cells using EZ1 instruments

Fully automated low- to medium-throughput purification of nucleic acids

For purification of total RNA from tissue and cells using EZ1 instruments

Download Safety Data Sheets for QIAGEN product components.

FAQ

Operation of the BioRobot EZ1 is so easy that no additional training other than the initial demo is needed. Operating the instrument requires only three major steps:

Insert the appropriate EZ1 Protocol Card, switch on the workstation, and, if necessary, select the appropriate protocol
Follow the instructions on the liquid-crystal display (LCD), which describes where the sample tubes, elution tubes, filter tips, and EZ1 Reagent Cartridges must be loaded and which indicates when to start the protocol run
Collect purified nucleic acids when the protocol run ends

FAQ ID -1239

No, the new EZ1 RNA Kits can only be used with updated protocols programmed on the new EZ1 RNA Card V2.0.

The new card contains all protocols available for the isolation of RNA on the BioRobot EZ1 with the new kits:

Purification of total RNA from cells using the EZ1 RNA Cell Mini Kit, with and without DNase digest.
Purification of total RNA from standard tissue using the EZ1 RNA Tissue Mini Kit, with and without DNase digest.
Purification of total RNA from any type of tissue using the EZ1 RNA Universal Tissue Kit, with and without DNase digest.

Likewise, the former EZ1 RNA Cell Mini Kit (cat. no. 958134), EZ1 RNA Tissue Mini Kit (cat. no. 959134) and EZ1 RNA Universal Tissue Kit (cat. no. 956136) are not compatible with the new EZ1 RNA Card (cat. no. 9015590).

FAQ ID -1025

Yes, please follow the Supplementary Protocol 'Isolation of total nucleic acids from animal and human tissues using the EZ1 RNA Tissue Mini Kit'.

FAQ ID -975

Protocols for nucleic acid purification are supplied on pre-programmed EZ1 Protocol Cards. These protocols provide both on-screen instructions for the operator and operating commands for the workstation. The range of kits and protocols for the EZ1 is continuously expanding to allow nucleic acid purification from a wide range of sample types. Please browse our Supplementary Protocol Section to find specialized EZ1 protocols for a large variety of clinical sample types, in addition to the standard sample types covered in our EZ1 handbooks.

FAQ ID -1241

Loading of sample tubes, elution tubes, filter-tips, and Reagent Cartridges onto the worktable of the BioRobot EZ1 is intuitive, easy and quick. Details about worktable setup are provided with each new protocol, and are also displayed in the liquid-crystal display (LCD) of the control panel when you start setup of the worktable.

FAQ ID -1243

No, prefilled Reagent Cartridges can be loaded onto the BioRobot EZ1 with their seals. During operation of the instrument, the piercing unit, a row of 6 metal spikes located behind the tip adapters, will puncture the foil of the cartridges, thereby exposing the reagents.

FAQ ID -1240

For RNA isolated on the BioRobot EZ1 and BioRobot M48:

The RNA can be directly applied to the Agilent Bioanalyzer, since it is being denatured in the final protocol steps of these isolation procedures.

For RNA prepared with all other QIAGEN RNA Isolation Products:

We recommend to denature the samples in a water bath for 2 min at 70°C, and then place them directly on ice prior to loading them onto the Agilent Bioanalyzer.

FAQ ID -528

Yes, please follow the User-Developed Protocol 'Purification of total RNA from paraffin-embedded sections using the EZ1 RNA Tissue Mini Kit' (MA33).

FAQ ID -976

Yes, please follow the Supplementary Protocol 'Purification of DNA from fungi using the EZ1 DNA Tissue Kit' (MA35).

FAQ ID -992

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12

No extra accessories need to be purchased for the BioRobot EZ1, as sample and elution tubes, as well as tips and tip holders are included with each EZ1 DNA or RNA isolation kit.

FAQ ID -1246